Anti-Plexin-A2 (extracellular) Antibody | Alomone Labs APR-082 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Plexin-A2 (extracellular) Antibody

catalog :

APR-082

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bautzová T, Hockley J, Pérez Berezo T, Pujo J, Tranter M, Desormeaux C, et al. 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein-coupled receptor D. Sci Signal. 2018;11: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:200).3,4. Anti-Plexin-A2 (extracellular) Antibody, preincubated with Plexin-A2 (extracellular) Blocking Peptide (#BLP-PR082).

image 2 :

Multiplex staining of Plexin-A2 and p75NTR in rat brain - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Plexin-A2 (extracellular) Antibody (#APR-082), (1:400) and mouse Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (#AN-170), (1:300). A. Sections of rat horizontal diagonal band (HDB) were stained for PLXNA2 (green). B. Same sections were stained for p75NTR (red). C. Merge of the two images reveals cells expressing both PLXNA2 and p75NTR (horizontal arrows), cells expressing only PLXNA2 (upwards pointing arrows) or cells expressing only p75NTR (downwards pointing arrows) can also be observed. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-082

SKU :

APR-082-CF_0.2 ml

Product Name :

Anti-Plexin-A2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70207

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR082

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2

Product Page - Scientific background :

The Plexin gene family is comprised of at least 9 members in 4 subfamilies. Plexins are widely expressed in neurons but have been also detected in epithelial cells1. Plexins closely interact with a large family of proteins called Semaphorins which control a variety of processes in the CNS including cell migration and axonal growth. Plexins and Semaphorins share a common feature of a conserved 500 amino acid "sema" domain2. The ectodomain of PLXNA2 is an N-terminal seven bladed β-propeller sema domain followed by a cysteine rich PSI (plexin-semaphorin-integrin) domain. PLXNA2 has four N-terminal domains and unlike semaphorins the sema domain of unliganded PLXNA2 does not homodimerize readily.Many of the functions of Class 3 semaphorins (Sema3A-Sema3G) are mediated by Plexin-A2 (PLXNA) and other A-plexins. Sema3 signaling depends on semaphorin interacting with one of the two Nrp co-receptors that associate with PLXNA to generate holoreceptors.PLXNA also interacts with semaphorin 6A (Sema6A). Before binding, the Sema6A ectodomain can be found in a 'face-to-face' homodimer arrangement, similar to the arrangement in Sema3, whereas PLXNA is in an unexpected 'head-on' homodimer arrangement. The structure of the Sema6A-PLXNA signaling complex is a 2:2 heterotetramer3.In prostate cancer TMPRSS:ERG gene fusion causes aberrant expression of the transcription factor ERG and the induction of cell migration and invasion. TMPRSS:ERG upregulates PLXNA expression and elevated PLXNA levels were found in prostate cancer cells. Levels of PLXNA were even higher in metastatic prostate cancer than in localized tumors suggesting PLXNA may play a role in the disease's development.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PLXNA2, PLXN2, Semaphorin receptor OCT

Short Description :

A Rabbit Polyclonal Antibody to Plexin-A2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Plexin-A2. Anti-Plexin-A2 (extracellular) Antibody (#APR-082) can be used in western blot and immunohistrochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting PLXNA2 in living cells. It has been designed to recognize PLXNA2 from rat, mouse and human samples.

Negative Control :

BLP-PR082

Positive Control :

Synonyms :

PLXNA2, PLXN2, Semaphorin receptor OCT

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PLXNA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage