Anti-Plexin-A1 (extracellular) Antibody | Alomone Labs APR-081 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Plexin-A1 (extracellular) Antibody

catalog :

APR-081

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Creighton B, Afriyie S, Ajit D, Casingal C, Voos K, Reger J, et al. Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A. elife. 2021;10: pubmed publisher
Cechmanek P, Hehr C, McFarlane S. Retinal Pigment Epithelium and Neural Retinal Progenitors Interact via Semaphorin 6D to Facilitate Optic Cup Morphogenesis. Eneuro. 2021;8: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain synaptosomal fractions: - 1,2. Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400).3,4. Anti-Plexin-A1 (extracellular) Antibody, preincubated with Plexin-A1 (extracellular) Blocking Peptide (#BLP-PR081).

image 2 :

Multiplex staining of GluN1 and Plexin-A1 in rat olfactory bulb - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody (#AGC-001-GP), (1:600) and rabbit Anti-Plexin-A1 (extracellular) Antibody (#APR-081), (1:400). A. GluN1 (green) is expressed in the glomeruli (horizontal arrows). B. PLXNA1 staining (red) in the same section, shows expression in the glomeruli (arrow) but also in other layers (vertical arrow) of the olfactory bulb. C. Merge of the two images showcases co-localization in the glomeruli vs. lack of co-localization in deeper layers of the olfactory bulb. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human Malme-3M melanomacell line lysate(lanes 2 and 4): - 12.Anti-TROY (TNFRSF19) (extracellular) Antibody(#ANT-033) (1:400).34. Anti-TROY (TNFRSF19) (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APR-081

SKU :

APR-081-CF_0.2 ml

Product Name :

Anti-Plexin-A1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UIW2

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR081

Homology :

Mouse, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human PLXNA1

Product Page - Scientific background :

The plexin family are transmembrane receptors, comprised four subfamilies: plexin-A, B, C, and D. In humans, three members of the plexin-A subfamily have been isolated: plexin-A1, A2, and A3. Plexin A1 is encoded by the PLXNA1 gene. These receptors are characterized by the presence of a conserved intracellular domain of about 600 amino acids known as the sex and plexins domain. This domain is strikingly conserved across the plexin family and consists of two blocks of high sequence conservation separated by a variable linker. The ectodomains of the plexin-A subfamily possess three repeats of the cysteine cluster/Met-related sequences.Plexins act as signal-transducing components containing transmembrane domain of six Gly residues constituting a GxGGGGG motif.Plexins act as functional receptors for members of the semaphorin family. Together they transmit repulsive axon guidance signals for semaphorins inside the axonal growth cone. The plexins act together with their co-receptors, neuropilins. Plexin-A1, in combination with neuropilins exhibits various functions in axon guidance, organogenesis, angiogenesis, tumorigenesis, and immune regulation1-3.Plexin-A1 also plays an important role in the developmental stages of chick heart by working as a receptor for one of the transmembrane semaphorins. In addition Plexin-A1 expressed in dendritic cells is involved in T-dendritic cell interactions in the immune system4.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PLXNA1, PLXN1, Semaphorin receptor NOV

Short Description :

A Rabbit Polyclonal Antibody to Plexin-A1

Long Description :

Anti-Plexin-A1 (extracellular) Antibody (#APR-081) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize PLXNA1 from mouse, rat and human samples.

Negative Control :

BLP-PR081

Positive Control :

Synonyms :

PLXNA1, PLXN1, Semaphorin receptor NOV

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PLXNA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage