Anti-Plexin-A1 (extracellular)-FITC Antibody | Alomone Labs APR-081-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Plexin-A1 (extracellular)-FITC Antibody

catalog :

APR-081-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Maimon R, Ankol L, Gradus Pery T, Altman T, Ionescu A, Weissova R, et al. A CRMP4-dependent retrograde axon-to-soma death signal in amyotrophic lateral sclerosis. EMBO J. 2021;40:e107586 pubmed publisher
Cechmanek P, Hehr C, McFarlane S. Retinal Pigment Epithelium and Neural Retinal Progenitors Interact via Semaphorin 6D to Facilitate Optic Cup Morphogenesis. Eneuro. 2021;8: pubmed publisher

image

image 1 :

Cell surface detection of PLXNA1 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of PLXNA1 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-FITC. Green line: Cells + Anti-Plexin-A1 (extracellular)-FITC Antibody (APR-081-F), (2.5 µg antibody/0.5x106 cells).

product information

CAT :

APR-081-F

SKU :

APR-081-F_50 mcl

Product Name :

Anti-Plexin-A1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UIW2

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR081

Homology :

Mouse, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human PLXNA1

Product Page - Scientific background :

The plexin family are transmembrane receptors, comprises four subfamilies- plexin-A, B, C, and D. In human, three members of the plexin-A subfamily have been isolated- plexin-A1, A2, and A3. Plexin A1 is encoded by the PLXNA1 gene.These receptors are characterized by the presence of a conserved intracellular domain of about 600 amino acids known as the sex and plexins domain. This domain is strikingly conserved across the plexin family and consists of two blocks of high sequence conservation separated by a variable linker. The ectodomains of the plexin-A subfamily possess three repeats of the cysteine cluster/Met-Related Sequences.Plexins act as signal-transducing components containing transmembrane domain of six Gly residues constituting a GxGGGGG motif.Plexins act as functional receptors for members of the semaphorin family. Together they transmit repulsive axon guidance signals for semaphorins inside the axonal growth cone. The plexins act together with their co-receptors, neuropilins. Plexin-A1, in combination with neuropilins exhibits various functions in axon guidance, organogenesis, angiogenesis, tumorigenesis, and immune regulation1-3.Plexin-A1 also plays an important role in the developmental stages of chick heart by working as a receptor for one of the transmembrane semaphorins. In addition Plexin-A1 expressed in dendritic cells is involved in T-dendritic cell interactions in the immune system4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

PLXNA1, PLXN1, Semaphorin receptor NOV

Short Description :

A Rabbit Polyclonal Antibody to Plexin-A1 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-Plexin-A1 (extracellular) Antibody (#APR-081) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PLXNA1 from mouse, rat, and human samples. Anti-Plexin-A1 (extracellular)-FITC Antibody (#APR-081-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

PLXNA1, PLXN1, Semaphorin receptor NOV

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PLXNA1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-081), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage