Anti-PEX5L (TRIP8b) Antibody | Alomone Labs APR-070 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PEX5L (TRIP8b) Antibody

catalog :

APR-070

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:4000; loading ...; fig 4c	Scherschel K, Bräuninger H, Mölders A, Erlenhardt N, Amin E, Jungen C, et al. Characterization of the HCN Interaction Partner TRIP8b/PEX5R in the Intracardiac Nervous System of TRIP8b-Deficient and Wild-Type Mice. Int J Mol Sci. 2021;22: pubmed publisher
	Zhang Y, Yin N, Sun A, Wu Q, Hu W, Hou X, et al. Transient Receptor Potential Channel 6 Knockout Ameliorates Kidney Fibrosis by Inhibition of Epithelial-Mesenchymal Transition. Front Cell Dev Biol. 2020;8:602703 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:400).3,4. Anti-PEX5L (TRIP8b) Antibody, preincubated with PEX5L/TRIP8b Blocking Peptide (#BLP-PR070).

image 2 :

Expression of PEX5-related protein in rat brain - Immunohistochemical staining of rat parietal cortex using Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:300). A. PEX5Rp (green) is labeled in dendritic and axonal profiles (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

image 3 :

Expression of PEX5-related protein in rat brain - Immunohistochemical staining of rat parietal cortex usingAnti-PEX5L (TRIP8b) Antibody(#APR-070) (1:300). A. PEX5Rp (green) is labeled in dendritic and axonal profiles (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.

product information

CAT :

APR-070

SKU :

APR-070-CF_0.2 ml

Product Name :

Anti-PEX5L (TRIP8b) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q925N3

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR070

Homology :

Mouse - identical; human - 13/15 amino acid residues identical; rabbit - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracelular.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein

Product Page - Scientific background :

PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein

Short Description :

A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (#APR-070) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.

Negative Control :

BLP-PR070

Positive Control :

Synonyms :

PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PEX5L

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage