Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody | Alomone Labs APR-068 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody

catalog :

APR-068

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse heart membranes (lanes 1 and 4), mouse lung lysate (lanes 2 and 5) and rat lung membranes (lanes 3 and 6): - 1-3. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068), (1:400).4-6. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody, preincubated with Prostacyclin Receptor/PTGIR (extracellular) Blocking Peptide (#BLP-PR068).

product information

CAT :

APR-068

SKU :

APR-068-CF_0.2 ml

Product Name :

Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43253

Applications :

IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR068

Homology :

Mouse - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR

Product Page - Scientific background :

Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30% of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Short Description :

A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)

Long Description :

Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (#APR-068) is a highly specific antibody directed against an extracellular epitope of the rat PGI2 receptor. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize PGI2 receptor from mouse, rat and human samples.

Negative Control :

BLP-PR068

Positive Control :

Synonyms :

Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGIR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage