Anti-Prostaglandin F2-α Receptor/PTGFR Antibody | Alomone Labs APR-067 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostaglandin F2-α Receptor/PTGFR Antibody

catalog :

APR-067

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6) lysates: - 1-3. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067), (1:200). 4-6. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody, preincubated with Prostaglandin F2-α Receptor/PTGFR Blocking Peptide (#BLP-PR067).

image 2 :

Expression of Prostaglandin F2-alpha receptor in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) (1:200), followed by goat anti-rabbit-AlexaFluor-488. PTGFR staining (green) in mouse hippocampal dentate gyrus is detected in cell bodies (arrows). Cell nuclei are stained with DAPI (blue). Mouse brain hippocampal dentate gyrus sections were obtained two hours following kainate acid intraventricular injection.

product information

CAT :

APR-067

SKU :

APR-067-CF_0.2 ml

Product Name :

Anti-Prostaglandin F2-α Receptor/PTGFR Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43118

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR067

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSQQHRQGRSHHLE, corresponding to amino acid residues 233 - 246 of rat Prostaglandin F2-α Receptor

Product Page - Scientific background :

Prostaglandins (PGs) are key lipid mediators derived from arachidonic acid that are involved in numerous physiological and pathological processes including inflammation and cardiovascular homeostasis. Each PG acts on its specific and distinct cell surface G-protein coupled receptor (GPCR) or peroxisome proliferator-activated receptor (PPAR)1.All prostaglandins are composed of a cyclopentanone nucleus with two side chains. Currently, three classes of prostaglandins are recognized, and these are categorized on the basis of the number of double bonds present within the prostaglandin molecule and on the fatty acid from which they are derived.Prostaglandin F2α (PGF2α) and its receptor (FP) are required for female reproductive functions such as luteolysis and parturition2. PGF2α was shown to be an important regulator of corpora luteal (CL) function, uterine contractility, ovulation, and embryo attachment in female swine. High affinity PGF2α receptors are present in the CL at all stages of the estrous cycle3.Apart from its pivotal role in parturition, PGF2α and its receptor are been implicated in blood pressure regulation4, atherosclerosis and other inflammation-related disorders5, cardiovascular diseases6 and bone development7. Prostaglandin F2α analogs were the first prostaglandin agonists introduced for glaucoma treatment, its mechanism of action includes reduction of intracellular pressure by increasing the uveoscleral outflow8.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor

Short Description :

A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor

Long Description :

Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize PTGFR from human, mouse, and rat samples.

Negative Control :

BLP-PR067

Positive Control :

Synonyms :

Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGFR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage