Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody | Alomone Labs APR-066 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody

catalog :

APR-066

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4): - 1,2. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066), (1:200).3,4. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody, preincubated with Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066).

product information

CAT :

APR-066

SKU :

APR-066-CF_0.2 ml

Product Name :

Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P43114

Applications :

IFC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR066

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RKTVLSKAIEKIK, corresponding to amino acid residues 336-348 of rat prostanoid EP4 receptor

Product Page - Scientific background :

E-type prostanoid receptor 4 (EP4) is responsible for mediating the effects of prostaglandin E2 (PGE2), an endogenous molecule involved in several biological processes such as pain, fever, regulation of vascular tone, renal function, mucosal integrity, inflammation, angiogenesis and tumor growth. In turn, EP4 receptor is involved in anti-inflammatory, anti-thrombotic, vasoprotective effects, adverse tumor-promoting and pro-angiogenic roles1,2.EP4 mRNA expression is detected in the gastrointestinal tract, uterus, hematopoietic tissues and skin. High expression is also found in most immune cell types. The EP receptors activate various signaling pathways. EP4 is a Gs coupled receptor, which activates adenylate cyclase and activates intracellular cyclic adenylyl monophosphate (cAMP) production1,2.The EP receptors contain an aspartate in the second transmembrane domain, which is involved in receptor-ligand interaction and conserved cysteine residues in the second and third extracellular domains, which form a disulfide bonds critical for stabilizing the receptor and for ligand binding3.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Prostaglandin E2 receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor

Short Description :

A Rabbit Polyclonal Antibody to Prostanoid EP4 Receptor

Long Description :

Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize EP4 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR066

Positive Control :

Synonyms :

Prostaglandin E2 receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGER4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage