Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody | Alomone Labs APR-065 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody

catalog :

APR-065

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Petersen C, Mahmood B, Badsted C, Dahlby T, Rasmussen H, Hansen M, et al. Possible predisposition for colorectal carcinogenesis due to altered gene expressions in normal appearing mucosa from patients with colorectal neoplasia. BMC Cancer. 2019;19:643 pubmed publisher
Durchschein F, Eherer A, Grill M, Sturm E, Pommer V, Langner C, et al. Involvement of EP2 and EP4 Receptors in Eosinophilic Esophagitis: A Pilot Study. Dig Dis Sci. 2019;: pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and pancreas (lanes 2 and 4) membranes: - 1,2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:200).3,4. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).

product information

CAT :

APR-065

SKU :

APR-065-CF_0.2 ml

Product Name :

Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34980

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR065

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RAPHWYASHMKTR, corresponding to amino acid residues 137-149 of mouse prostanoid EP3 receptor

Product Page - Scientific background :

Prostaglandin is a small molecule produced from arachidonic acid by the enzymes COX1, COX2 and PG synthase. PGE2 regulates various factors of inflammatory response, promoting local vasodilatation and attracting immune system cells to the site of the inflammation.Prostanoid EP3 receptor, a member of the G-protein coupled receptor superfamily, is one of four PGE2 receptors (EP1-EP4) and binds PGE2 with a higher affinity than other receptors of its class. Unlike EP2 and EP4, EP3 is not coupled to Gs and lacks cAMP activating functions. In contrast, most of its splice variants couple with Gi and inhibit adenylate cyclase activity1. EP3 binds prostaglandin in a GTP dependent manner. One of the receptor's extracellular domains, ECII, can alter GTP effect on agonist binding and thus the receptor's downstream intracellular signaling. ECII causes receptor conformational changes2.EP3 plays a role in pulmonary hypertension (PAH) pathophysiology. It is hypothesized that EP3 levels are upregulated in pulmonary arterial smooth muscle cells and in human distal pulmonary arteries in response to hypoxia. Blocking agents for the EP3 receptor may prove as a useful strategy in the treatment of PAH3.EP3 is also involved in the cell growth, invasion and migration of many tumor types including bladder cancer. EP3 has been found to be significantly less expressed in muscular and non-muscular invasive bladder cells than in normal urothelial cells4.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Prostaglandin E2 receptor EP3 subtype, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor

Short Description :

A Rabbit Polyclonal antibody to the Prostanoid EP3 Receptor

Long Description :

Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize EP3 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR065

Positive Control :

Synonyms :

Prostaglandin E2 receptor EP3 subtype, PGE2 receptor EP3 subtype, Prostanoid EP3 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGER3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage