Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody

catalog :

APR-064

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Durchschein F, Eherer A, Grill M, Sturm E, Pommer V, Langner C, et al. Involvement of EP2 and EP4 Receptors in Eosinophilic Esophagitis: A Pilot Study. Dig Dis Sci. 2019;: pubmed publisher
Cantaut Belarif Y, Antri M, Pizzarelli R, Colasse S, Vaccari I, Soares S, et al. Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord. J Cell Biol. 2017;216:2979-2989 pubmed publisher
Bonfill Teixidor E, Otxoa de Amezaga A, Font Nieves M, Sans Fons M, Planas A. Differential expression of E-type prostanoid receptors 2 and 4 in microglia stimulated with lipopolysaccharide. J Neuroinflammation. 2017;14:3 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), rat stomach (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates: - 1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).

image 2 :

Expression of Prostanoid EP2 receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:100). Prostanoid EP2 receptor staining (red) is detected in Purkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.

image 3 :

Cell surface detection of Prostanoid EP2 receptor in live intact human Jurkat T cell leukemia cell line: - ___Unstained cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.___Cells +Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody(#APR-064) (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.

product information

CAT :

APR-064

SKU :

APR-064-CF_0.2 ml

Product Name :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62928

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR064

Homology :

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor

Product Page - Scientific background :

Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with an extracellular N-terminal tail and an intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles of the eye3.PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4.The EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor

Short Description :

A Rabbit Polyclonal Antibody to Prostanoid EP2 Receptor

Long Description :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize EP2 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR064

Positive Control :

Synonyms :

Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGER2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage