Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody | Alomone Labs APR-064-PE product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody

catalog :

APR-064-PE

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of PTGER2 by direct flow cytometry in live intact mouse J774 macrophage cell line:

image 2 :

Cell surface detection of PTGER2 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:

product information

CAT :

APR-064-PE

SKU :

APR-064-PE_15 mcl

Product Name :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62928

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR064

Homology :

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 μl or 50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

R-Phycoerythrin (R-PE).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor

Product Page - Scientific background :

Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with an extracellular N-terminal tail and an intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles of the eye3.PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4.The EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor

Short Description :

A Rabbit polyclonal antibody to Prostaglandin E Receptor EP2/PTGER2 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.

Long Description :

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize EP2 receptor from rat, mouse, and human samples. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular)-PE Antibody (#APR-064-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-PE

Positive Control :

Synonyms :

Prostaglandin E2 receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGER2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-064), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage