Anti-CRTH2/GPR44 (extracellular) Antibody | Alomone Labs APR-062 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CRTH2/GPR44 (extracellular) Antibody

catalog :

APR-062

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200).2. Anti-CRTH2/GPR44 (extracellular) Antibody, preincubated with CRTH2/GPR44 (extracellular) Blocking Peptide (#BLP-PR062).

image 2 :

Expression of DP2 receptor (CRTH2/GPR44) in rat medial septum and mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. DP2 receptor staining (green) in rat brain sections is detected in neuron-like profiles (arrows) in the medial septum/diagonal band. B. DP2 receptor staining (green) in mouse brain is observed in Purkinje cells (horizontal arrow) and in a subset of neurons in the granule layer (GL, vertical arrow). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-062

SKU :

APR-062-CF_0.2 ml

Product Name :

Anti-CRTH2/GPR44 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6XKD3

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR062

Homology :

Mouse - identical Will not recognize DP2 receptor from human samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RAHSVTTLRQLASR, corresponding to amino acid residues 270-283 of rat PTGDR2

Product Page - Scientific background :

Prostanoids are potent oxygenated lipid molecules that contribute to a wide variety of physiological responses and pathological processes. There are currently five primary bioactive types of prostanoids: PGD2, PGE2, PGF2R, PGI2, and thromboxane A2 (TXA2).All prostanoid receptors belong to the G-protein coupled receptor superfamily. Unlike other receptors of this family the Prostaglandin D2 receptor (also known as CRTH2, GPR44) is structurally distinct and belongs to the family of leukocyte chemoattractant receptors.The DP2 receptor is expressed in Th2 cells, basophils, and eosinophils, and it mediates the chemotaxis of these cells in response to PGD2. The ability of PGD2 to promote eosinophil accumulation in the airways is mimicked by selective DP2 agonists but not DP1 agonists.Much effort has been directed toward developing DP2 agonists and antagonists. Indomethacin and ramatroban, which act, respectively, as an agonist and an antagonist, were the first nonprostanoid compounds to be identified as DP2 ligands. These two compounds have frequently used as leads for identifying new selective compounds1.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294

Short Description :

A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)

Long Description :

Anti-CRTH2/GPR44 (extracellular) Antibody (#APR-062) is a highly selective antibody directed against an epitope of the rat Prostanoid DP2 receptor. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize DP2 receptor from mouse and rat samples. It is unlikely to recognize the receptor from human samples.

Negative Control :

BLP-PR062

Positive Control :

Synonyms :

Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTGDR2

AB Specifictiy :

Will not recognize DP2 receptor from human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage