Anti-Platelet-activating factor receptor (extracellular) Antibody

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Platelet-activating factor receptor (extracellular) Antibody

catalog :

APR-055

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:500). 3-4. Anti-Platelet-activating factor receptor (extracellular) Antibody, preincubated with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055).

image 2 :

Expression of Platelet-activating factor receptor in rat substantia nigra pars compacta (SNC). - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. PAFR immunoreactivity (green) appears in SNC neurons (arrows). B. Pre-incubation of the antibody with Platelet-activating factor receptor (extracellular) Blocking Peptide (BLP-PR055), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-055

SKU :

APR-055-CF_0.2 ml

Product Name :

Anti-Platelet-activating factor receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62035

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR055

Homology :

Rat - identical, Human - 13 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR

Product Page - Scientific background :

Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PTAFR, PAFR

Short Description :

A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)

Long Description :

Anti-Platelet-activating factor receptor (extracellular) Antibody (#APR-055) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PAFR from rat, mouse and human samples.

Negative Control :

BLP-PR055

Positive Control :

Synonyms :

PTAFR, PAFR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTAFR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage