Anti-PrRP Receptor (GPR10) Antibody | Alomone Labs APR-054 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PrRP Receptor (GPR10) Antibody

catalog :

APR-054

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and PC-12 cells (lines 4 and 8): - 1-4. Anti-PrRP Receptor (GPR10) Antibody (#APR-054), (1:1000).5-8. Anti-PrRP Receptor (GPR10) Antibody, preincubated with PrRP Receptor/GPR10 Blocking Peptide (#BLP-PR054).

image 2 :

Peptide (C)RVSVKLRNRVVPGS corresponding to amino acid residues 247-260 of rat PRLHR (AccessionQ64121). 3rdintracellular loop.

product information

CAT :

APR-054

SKU :

APR-054-CF_0.2 ml

Product Name :

Anti-PrRP Receptor (GPR10) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64121

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR054

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR

Product Page - Scientific background :

Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.PrRP is implicated in the regulation of the hypothalamic-pituitary-adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Short Description :

A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (#APR-054) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.

Negative Control :

BLP-PR054

Positive Control :

Synonyms :

Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PRLHR

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage