Anti-PTH2R (extracellular) Antibody | Alomone Labs APR-052 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PTH2R (extracellular) Antibody

catalog :

APR-052

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-PTH2R (extracellular) Antibody (#APR-052), (1:600).3,4. Anti-PTH2R (extracellular) Antibody, preincubated with PTH2R (extracellular) Blocking Peptide (#BLP-PR052).

image 2 :

Peptide CFLQPDINIGKQE corresponding to amino acid residues 125-137 of rat PTH2R (AccessionP70555). Extracellular N-terminus.

image 3 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 12. Anti-EphA3 (extracellular) Antibody (#AER-013) (1:200).34. Anti-EphA3 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APR-052

SKU :

APR-052-CF_0.2 ml

Product Name :

Anti-PTH2R (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70555

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR052

Homology :

Mouse - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat PTH2R

Product Page - Scientific background :

PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRH, VIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4.Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Short Description :

A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor

Long Description :

Anti-PTH2R (extracellular) Antibody (#APR-052) is a highly specific antibody directed against an extracellular epitope of the rat Parathyroid hormone 2 receptor. The antibody can be used in western blot analysis. It has been designed to recognize PTH2 receptor from human, rat and mouse samples.

Negative Control :

BLP-PR052

Positive Control :

Synonyms :

Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTH2R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage