Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs APR-052-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody

catalog :

APR-052-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Multiplex staining of Parathyroid hormone 2 receptor and Vesicular glutamate transporter 2 in mouse hippocampus - Immunohistochemical staining of mouse hippocampal CA3 region frozen sections using Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody (#APR-052-AG), (1:60) and Anti-VGLUT2-ATTO Fluor-594 Antibody (#AGC-036-AR), (1:60). A. PTH2 receptor staining (green). B. VGLUT2 staining (red) in same section. C. Merge of A and B suggests extensive localization of PTH2R on glutamatergic neurons.

image 2 :

Alomone Labs human proBDNF-Biotin induces phosphorylation of ERK1/2 (p42/44 MAPK) in TrkB-expressing HEK 293 cells. - Cells were stimulated with 02 10 and 50 ng/mlhuman proBDNF-biotin(#B-256-B) and with 50 ng/mlRecombinant human proBDNF (cleavage resistant) protein(#B-256) as indicated. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-ERK1/2.

product information

CAT :

APR-052-AG

SKU :

APR-052-AG_50 mcl

Product Name :

Anti-PTH2R (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70555

Applications :

IF IHC

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR052

Homology :

Mouse - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat PTH2R

Product Page - Scientific background :

PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRH, VIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4. Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Short Description :

A Rabbit Polyclonal Antibody to Parathyroid Hormone 2 Receptor Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-PTH2R (extracellular) Antibody (#APR-052) is a highly specific antibody directed against an extracellular epitope of the rat Parathyroid hormone 2 receptor. The antibody can be used in western blot analysis. It has been designed to recognize PTH2 receptor from human, rat and mouse samples. Anti-PTH2R (extracellular)-ATTO-488 Antibody (#APR-052-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-PTH2R (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

Parathyroid hormone 2 receptor, PTH2 receptor, PTHR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTH2R

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody,#APR-052), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage