Anti-PTH1R (extracellular) Antibody | Alomone Labs APR-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PTH1R (extracellular) Antibody

catalog :

APR-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wang X, Yuan X, Su Y, Hu J, Ji Q, Fu S, et al. Targeting Purinergic Receptor P2RX1 Modulates Intestinal Microbiota and Alleviates Inflammation in Colitis. Front Immunol. 2021;12:696766 pubmed publisher

image

image 1 :

Western blot analysis of HepG2 (lanes 1 and 4), mouse liver (lanes 2 and 5) and rat liver (lanes 3 and 6) lysates: - 1-3. Anti-PTH1R (extracellular) Antibody (#APR-051), (1:200).4-6. Anti-PTH1R (extracellular) Antibody, preincubated with PTH1R (extracellular) Blocking Peptide (#BLP-PR051).

image 2 :

Expression of Parathyroid hormone receptor 1 in rat brain - Immunohistochemical staining of rat dorsal Raphe nucleus using Anti-PTH1R (extracellular) Antibody (#APR-051). A. PTH1R (red) staining is detected in Raphe neurons (arrows). B. Nucleus staining using DAPI as the counterstain (blue). C. Merged images of A and B.

image 3 :

Expression of Parathyroid hormone receptor 1 in rat U-87 MG cells - Cell surface detection of Parathyroid hormone receptor 1in intact living rat U-87 MG cells. A. Extracellular staining of cells usingAnti-PTH1R (extracellular) Antibody(#APR-051) (1:50) (green). B. Merge of A with live view of the cells.

product information

CAT :

APR-051

SKU :

APR-051-CF_0.2 ml

Product Name :

Anti-PTH1R (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25961

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR051

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DAVLYSGFTLDEAER, corresponding to amino acid residues 241-255 of rat PTH1R

Product Page - Scientific background :

The parathyroid hormone receptor type 1 (PTHR1) is bound and activated by two peptide hormones: the parathyroid hormone (PTH) secreted by the parathyroid gland and by parathyroid hormone related peptide (PTHrP) which is produced locally in many tissues. Although these two peptides bind the same receptor, they exert different actions. PTH regulates the concentrations of Ca2+, phosphate ions and vitamin D in the blood, stimulates bone formation/resorption and is distributed to the kidneys where it regulates Ca2+ and phosphate transport, and is also detected in the liver1-3. PTHrP, on the other hand, plays a critical role early in development of bone, heart, mammary glands and other tissues, and is detected in the circulation only during lactation and secreted by some tumors1,2,4,5. PTHR1 is obviously localized to all areas and tissues where PTH and PTHrP are known to exert their effects (i.e. bone, kidney, blood, heart, etc.).PTHR1 belongs to the superfamily of G-protein coupled receptors. Like all members, PTHR1 has seven transmembrane spanning domains, an extracellular N-terminus and an intracellular C-terminal tail. Upon activation, the receptor couples to either Gs and Gq thereby activating adenylate cyclase and phospholipase C (PLC) respectively. In osteosarcoma cells, PTHR1 can also couple Gα2,6,7.PTH is being used today to treat osteoporosis, as a factor promoting bone formation. Mutations in PTHR1 are associated with Jansen's disease (hypercalcemia and dwarfism) and Eiken syndrome (cartilage tumors of bone)2,8-10.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1

Short Description :

A Rabbit Polyclonal Antibody to Parathyroid Hormone 1 Receptor

Long Description :

Anti-PTH1R (extracellular) Antibody (#APR-051) is a highly specific antibody directed against an epitope of the rat Parathyroid hormone 1 receptor. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize PTH1R from human, mouse, and rat samples.

Negative Control :

BLP-PR051

Positive Control :

Synonyms :

Parathyroid hormone 1 receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR, PTHR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PTH1R

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage