Anti-Prokineticin Receptor 2 (extracellular) Antibody | Alomone Labs APR-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prokineticin Receptor 2 (extracellular) Antibody

catalog :

APR-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Morris E, Patton A, Chesham J, Crisp A, Adamson A, Hastings M. Single-cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin-driven circadian network. EMBO J. 2021;40:e108614 pubmed publisher
Moschetti G, Amodeo G, Maftei D, Lattanzi R, Procacci P, Sartori P, et al. Targeting prokineticin system counteracts hypersensitivity, neuroinflammation, and tissue damage in a mouse model of bortezomib-induced peripheral neuropathy. J Neuroinflammation. 2019;16:89 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (1:200).2. Anti-Prokineticin Receptor 2 (extracellular) Antibody, preincubated with Prokineticin Receptor 2 (extracellular) Blocking Peptide (#BLP-PR042).

image 2 :

Expression of Prokineticin receptor 2 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in small, medium and large DRG neurons. Hoechst 33342 is used as the counterstain (blue).

image 3 :

Expressionof Prokineticin receptor 2 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections usingAnti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in small medium and large DRG neurons. Hoechst 33342 is used as the counterstain (blue).

product information

CAT :

APR-042

SKU :

APR-042-CF_0.2 ml

Product Name :

Anti-Prokineticin Receptor 2 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R415

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR042

Homology :

Mouse - 11/13 amino acid residues identical; human - 9/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat PROKR2

Product Page - Scientific background :

The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1PKR2 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.The distribution of PKR2 is relatively restricted with high expression levels in the brain, spinal cord and dorsal root ganglions, in organs of the reproductive system and in endocrine tissues such as the thyroid, pituitary and adrenal glands.1The PK2 ligand has been shown to be involved in the regulation of circadian rhythms of physiological and behavioral processes in mammals, probably through signaling via PKR2 which is highly expressed in the suprachiasmatic nucleus (SCN), an area of the brain that controls circadian rhythm processes.2In addition, loss-of-function mutations in the PKR2 gene have been associated with Kallmann Syndrome, a condition characterized by idiopathic hypogonadotropic hypogonadism (IHH) in combination with anosmia, a compromised sense of smell.3

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E

Short Description :

A Rabbit Polyclonal Antibody to Prokineticin Receptor 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 2. The epitope is specific for PROKR2 and will not cross-react with the closely related PROKR1. Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042) can be used in western blot and immunohistochemical applications. It has been designed to recognize PROKR2 from rat, mouse, and human samples.

Negative Control :

BLP-PR042

Positive Control :

Synonyms :

PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PROKR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage