Anti-Prokineticin Receptor 1 (extracellular) Antibody | Alomone Labs APR-041 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Prokineticin Receptor 1 (extracellular) Antibody

catalog :

APR-041

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Huang C, Zhang W, Sun A, Zhang X, Guo J, Ji R, et al. Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2/PKR1 Pathway. Oxid Med Cell Longev. 2020;2020:7075836 pubmed publisher
Li Y, Zhou T, Su Y, Hu Z, Wei J, Wang W, et al. Prokineticin 2 overexpression induces spermatocyte apoptosis in varicocele in rats. Asian J Androl. 2020;22:500-506 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:200).2. Anti-Prokineticin Receptor 1 (extracellular) Antibody, preincubated with Prokineticin Receptor 1 (extracellular) Blocking Peptide (#BLP-PR041).

image 2 :

Expression of Prokineticin receptor 1 in rat DRG - Immunohistochemical staining of frozen rat dorsal root ganglion (DRG) sections using Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041), (1:100), followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. PK-R1 (red) is expressed in DRG neurons. Cell nuclei are visualized with Hoechst 33342 (blue).

image 3 :

Western blot analysis of rat brain membranes: - 1.Anti-Prokineticin Receptor 1 (extracellular)Antibody (#APR-041) (1:200).2. Anti-Prokineticin Receptor 1 (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

APR-041

SKU :

APR-041-CF_0.2 ml

Product Name :

Anti-Prokineticin Receptor 1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R416

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR041

Homology :

Mouse - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat PROKR1

Product Page - Scientific background :

The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described.1PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain, particularly in olfactory regions, as well as in dorsal root ganglion (DRG) neurons.The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ

Short Description :

A Rabbit Polyclonal Antibody to Prokineticin Receptor 1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 1. The epitope is specific for PROKR1 and will not cross-react with the closely related PROKR2. Anti-Prokineticin Receptor 1 (extracellular) Antibody (#APR-041) can be used in western blot, immunohistochemistry and flow cytometry applications, and recognizes PROKR1 from rat and mouse samples. The antibody will not recognize the receptor from human samples.

Negative Control :

BLP-PR041

Positive Control :

Synonyms :

PROKR1, PKR1, G-protein coupled receptor 73, Gpr73, Gpr73a, G-protein coupled receptor ZAQ

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PROKR1

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage