Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody | Alomone Labs APR-034-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody

catalog :

APR-034-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of PAR-4 in live intact human MEG-01 megakaryoblastic leukemia cells: Cell surface detection of PAR-4 in live intact human MEG-01 megakaryoblastic leukemia cells: Black line: Cells alone. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody (#APR-034-F), 5µg.

image 2 :

Cell surface detection ofPAR-4 in live intact human MEG-01 megakaryoblastic leukemia cells: - ___Cells alone.___Cells + rabbit IgG isotype control-FITC.___Cells + Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody(#APR-034-F) 5g.

image 3 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5) human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6) human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8): - 1-4. Anti-EphA3 (extracellular) Antibody (#AER-013) (1:200).5-8. Anti-EphA3 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APR-034-F

SKU :

APR-034-F_50 mcl

Product Name :

Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96RI0

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR034

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4 Cys 149 was replaced with Ser.

Product Page - Scientific background :

Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsin.1-3 PAR-4 can be also cleaved and activated by other proteases such as cathepsin G.The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cβ through the Gαq pathway.1-3Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation.PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function.1-3

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3

Short Description :

A Rabbit Polyclonal Antibody to Protease-Activated Receptor-4 Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034) is a highly specific antibody directed against an extracellular epitope of human protease-activated receptor-4. The antibody can be used in western blot analysis, immunohistochemical, immunocytochemical and flow cytometry applications. It has been designed to recognized PAR-4 from human, mouse and rat samples. Anti-PAR4 (F2RL3) (extracellular)-FITC Antibody (#APR-034-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

F2RL3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-034), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage