antibody

Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody

APR-031-F

polyclonal

domestic rabbit

FITC

NA

APR-031-F_15 mcl

Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody

Antibodies

Antibodies

Polyclonal

P25116

FC LCI

Human

Rabbit

BLP-PR031

Monkey - identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Extracellular, N-terminus

Fluorescein isothiocyanate (FITC).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1

Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin.1-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others.The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby "disarming" the PAR.1-3PAR-1 signals through several G proteins including Gαq, Gαi, and Gα12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac signaling, and MAPK activation.1-3PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestinal epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation.1-3PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic signals through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.4

FC LCI

Alomone Labs

Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R

A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC

Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031) is a highly specific antibody directed against an epitope of human protease-activated receptor-1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize PAR-1 from human samples. Anti-Human PAR1 (F2R) (extracellular)-FITC Antibody (#APR-031-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

RIC-001-F

NA

Protease-activated receptor-1, PAR-1, Thrombin receptor, Coagulation factor II receptor, CF2R

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

F2R

Won’t recognize PAR-1 from mouse and rat samples.

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #APR-031), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information