Anti-P2X5 Receptor (extracellular) Antibody | Alomone Labs APR-027 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X5 Receptor (extracellular) Antibody

catalog :

APR-027

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Gou X, Hu T, Gou Y, Li C, Yi M, Jia M. Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking. Channels (Austin). 2021;15:262-272 pubmed publisher
Le Q, Kim J, Kim K, Van Vu A, Woo S. Distinct shear-induced Ca2+ signaling in the left and right atrial myocytes: Role of P2 receptor context. J Mol Cell Cardiol. 2020;143:38-50 pubmed publisher
Solís Chagoyán H, Flores Soto E, Valdés Tovar M, Cercós M, Calixto E, Montaño L, et al. Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells. Stem Cells Int. 2019;2019:2728786 pubmed publisher

image

image 1 :

Western blot analysis of rat heart (lanes 1 and 3) and mouse heart (lanes 2 and 4): - 1,2. Anti-P2X5 Receptor (extracellular) Antibody (#APR-027), (1:400).3,4. Anti-P2X5 Receptor (extracellular) Antibody, preincubated with P2X5 Receptor (extracellular) Blocking Peptide (#BLP-PR027).

image 2 :

Western blot analysis of rat heart (lanes 1 and 3) and mouse heart (lanes 2 and 4): - 12.Anti-P2X5 Receptor (extracellular)Antibody (#APR-027) (1:400).34. Anti-P2X5 Receptor (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Cell surface detection of CD73 in live intact human Jurkat T-cell leukemia cells: - ___ Cells.___ Cells + goat-anti-rabbit-FITC.___ Cells +Anti-CD73 (extracellular) Antibody (#ANT-066) (5 g) + goat-anti-rabbit-FITC.

product information

CAT :

APR-027

SKU :

APR-027-CF_0.2 ml

Product Name :

Anti-P2X5 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51578

Applications :

IFC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR027

Homology :

Mouse -12/13 amino acid residues; human - 10/13 amino acid residues

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor

Product Page - Scientific background :

The P2X5 receptor (P2X5R) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.1,2,3 All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The P2X5R forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.4,6 The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia. The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland. Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.7-10

Applications may also work in :

IFC LCI WB

Supplier :

Alomone Labs

Target :

P2RX5, P2X purinoceptor 5

Short Description :

A Rabbit Polyclonal Antibody to P2X5 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat P2X5 receptor. Anti-P2X5 Receptor (extracellular) Antibody (#APR-027) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal to detect the receptor in living cells. It has been designed to recognize P2RX5 from mouse, rat and human samples.

Negative Control :

BLP-PR027

Positive Control :

Synonyms :

P2RX5, P2X purinoceptor 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage