Anti-P2X3 Receptor (extracellular) Antibody | Alomone Labs APR-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X3 Receptor (extracellular) Antibody

catalog :

APR-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Dan Y, Guo H, Zheng C, Wu B, Guo J, Li G. Neferine alleviates P2X3 receptor in rat dorsal root ganglia mediated neuropathic pain. Neurosci Res. 2021;170:265-272 pubmed publisher
Katayama P, Abdala A, Charles I, Pijacka W, Salgado H, Gever J, et al. P2X3 receptor antagonism reduces the occurrence of apnoeas in newborn rats. Respir Physiol Neurobiol. 2020;277:103438 pubmed publisher
Leng C, Chen L, Li C. Alteration of P2X1-6 receptor expression in retrograde Fluorogold-labeled DRG neurons from rat chronic neuropathic pain model. Biomed Rep. 2019;10:225-230 pubmed publisher
Gu Y, Wang C, Li G, Huang L. EXPRESS: F-actin links Epac-PKC signaling to purinergic P2X3 receptors sensitization in dorsal root ganglia following inflammation. Mol Pain. 2016;12: pubmed publisher

image

image 1 :

Western blot analysis of rat DRG: - 1. Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:200).2. Anti-P2X3 Receptor (extracellular) Antibody, preincubated with P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026).

image 2 :

Expression of P2RX3 in rat DRG - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), followed by anti-rabbit-AlexaFluor-594 secondary antibody. P2RX3 staining (red) appears in neuronal cell bodies. DAPI was used as the counter stain (blue). A. X20 magnification. B. X40 magnification.

image 3 :

Expression of P2RX3 in rat PC12 cells - Cell surface detection ofP2RX3 in intact living rat pheochromocytoma (PC12) cells.A. Extracellular staining withAnti-P2X3 Receptor (extracellular) Antibody (#APR-026) (1:50 red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI was used as the counter stain (blue). C. Merge images of A and B.

product information

CAT :

APR-026

SKU :

APR-026-CF_0.2 ml

Product Name :

Anti-P2X3 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49654

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR026

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor

Product Page - Scientific background :

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2RX3, P2X purinoceptor 3

Short Description :

A Rabbit Polyclonal Antibody to P2X3 Receptor

Long Description :

Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR026

Positive Control :

Synonyms :

P2RX3, P2X purinoceptor 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage