Anti-P2X2 Receptor (extracellular) Antibody | Alomone Labs APR-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X2 Receptor (extracellular) Antibody

catalog :

APR-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:400; fig 8	Anastasiades P, Marques Smith A, Lyngholm D, Lickiss T, Raffiq S, KÃ¤tzel D, et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun. 2016;7:10584 pubmed publisher
	Godoy P, Mennickent D, Cuchillo Ibanez I, Ramírez Molina O, Silva Grecchi T, Panes Fernández J, et al. Increased P2×2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer's disease. Biomed Pharmacother. 2021;142:111968 pubmed publisher
	Leng C, Chen L, Li C. Alteration of P2X1-6 receptor expression in retrograde Fluorogold-labeled DRG neurons from rat chronic neuropathic pain model. Biomed Rep. 2019;10:225-230 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat pheochromocytoma PC12 cells (lanes 3 and 6): - 1-3. Anti-P2X2 Receptor (extracellular) Antibody (#APR-025), (1:200).4-6. Anti-P2X2 Receptor (extracellular) Antibody, preincubated with P2X2 Receptor (extracellular) Blocking Peptide (#BLP-PR025).

image 2 :

Peptide (C)KSDYLKHSTFDQDS corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (AccessionP49653). Extracellular.

image 3 :

Expression of P2RX2 in rat PC12 cells - Cell surface detection ofP2RX2 in intact living rat pheochromocytoma cell line (PC12). A. Extracellular staining of cells withAnti-P2X2 Receptor (extracellular) Antibody (#APR-025) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Staining of cells with DAPI used as the counterstain. C. Merged images of A and B.

product information

CAT :

APR-025

SKU :

APR-025-CF_0.2 ml

Product Name :

Anti-P2X2 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49653

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR025

Homology :

Rat, mouse - 13/14 amino acid residues identicalWill not recognize human P2X2

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S)

Product Page - Scientific background :

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

P2X purinoceptor 2, P2RX2

Short Description :

A Rabbit Polyclonal Antibody to P2X2 Receptor

Long Description :

Anti-P2X2 Receptor (extracellular) Antibody (#APR-025) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X2 Receptor from mouse, and rat samples. It is not suited to recognize human P2X2.

Negative Control :

BLP-PR025

Positive Control :

Synonyms :

P2X purinoceptor 2, P2RX2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage