Anti-P2X4 Receptor (extracellular)-PE Antibody | Alomone Labs APR-024-PE product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X4 Receptor (extracellular)-PE Antibody

catalog :

APR-024-PE

clonality :

polyclonal

host :

domestic rabbit

conjugate :

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Lalisse S, Hua J, Lenoir M, Linck N, Rassendren F, Ulmann L. Sensory neuronal P2RX4 receptors controls BDNF signaling in inflammatory pain. Sci Rep. 2018;8:964 pubmed publisher

image

image 1 :

Cell surface detection of P2X4 Receptor by direct flow cytometry in live intact mouse BV-2 microglia cells: Cell surface detection of P2X4 Receptor by direct flow cytometry in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + Rabbit IgG isotype control-PE. Green line: Cells + Anti-P2X4 Receptor (extracellular)-PE Antibody (#APR-024-PE), (2.5µg).

product information

CAT :

APR-024-PE

SKU :

APR-024-PE_50 mcl

Product Name :

Anti-P2X4 Receptor (extracellular)-PE Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51577

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR024

Homology :

Mouse - 12/13 amino acid residues identical;human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular

Label :

R-Phycoerythrin (R-PE).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor

Product Page - Scientific background :

The P2X purinergic receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop.All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

P2RX4, P2X purinoceptor 4, Purinergic Receptor P2X4, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X4 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)

Long Description :

Anti-P2X4 Receptor (extracellular) Antibody (#APR-024) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can be used to detect the protein in living cells. It has been designed to recognize P2X4 from human, mouse, and rat samples. Anti-P2X4 Receptor (extracellular)-PE Antibody (#APR-024-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-PE

Positive Control :

Synonyms :

P2RX4, P2X purinoceptor 4, Purinergic Receptor P2X4, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX4

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-024), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage