Anti-P2X1 Receptor (extracellular) Antibody | Alomone Labs APR-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X1 Receptor (extracellular) Antibody

catalog :

APR-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Wang X, Yuan X, Su Y, Hu J, Ji Q, Fu S, et al. Targeting Purinergic Receptor P2RX1 Modulates Intestinal Microbiota and Alleviates Inflammation in Colitis. Front Immunol. 2021;12:696766 pubmed publisher
Iguchi N, Malykhina A, Wilcox D. Early life voiding dysfunction leads to lower urinary tract dysfunction through alteration of muscarinic and purinergic signaling in the bladder. Am J Physiol Renal Physiol. 2018;315:F1320-F1328 pubmed publisher
Wright A, Mahaut Smith M, Symon F, Sylvius N, Ran S, Bafadhel M, et al. Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients. J Immunol. 2016;196:4877-84 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: - 1,2. Anti-P2X1 Receptor (extracellular) Antibody (#APR-022), (1:200).3,4. Anti-P2X1 Receptor (extracellular) Antibody, preincubated with P2X1 Receptor (extracellular) Blocking Peptide (#BLP-PR022).

image 2 :

Expression of P2X1 Receptor in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) Antibody (#APR-022). A. Most of P2RX1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.

image 3 :

Cell surface detection of P2RX1 in live MEG-O1 cells: - ___Unstained cells.___Cells +Anti-P2X1 Receptor (extracellular)Antibody (#APR-022) (10 g antibody/1x106cells).

product information

CAT :

APR-022

SKU :

APR-022-CF_0.2 ml

Product Name :

Anti-P2X1 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51575

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR022

Homology :

Rat, mouse - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor

Product Page - Scientific background :

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini two membrane-spanning domains and a large extracellular loop.All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex4-9.The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous systems, while the P2X7 receptor is predominantly found in cells of the immune system4. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis3,4,10,11.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2RX1, P2X purinoceptor 1

Short Description :

A Rabbit Polyclonal Antibody to P2X1 Receptor

Long Description :

Anti-P2X1 Receptor (extracellular) Antibody (#APR-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR022

Positive Control :

Synonyms :

P2RX1, P2X purinoceptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage