Anti-P2Y1 Receptor (extracellular) Antibody | Alomone Labs APR-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y1 Receptor (extracellular) Antibody

catalog :

APR-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; tbl 1	Brown I, McClain J, Watson R, Patel B, Gulbransen B. Enteric glia mediate neuron death in colitis through purinergic pathways that require connexin-43 and nitric oxide. Cell Mol Gastroenterol Hepatol. 2016;2:77-91 pubmed
	Palygin O, Klemens C, Isaeva E, Levchenko V, Spires D, Dissanayake L, et al. Characterization of purinergic receptor 2 signaling in podocytes from diabetic kidneys. iScience. 2021;24:102528 pubmed publisher
	Woehrle T, Ledderose C, Rink J, Slubowski C, Junger W. Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation. Purinergic Signal. 2019;: pubmed publisher
	Mitchell R, Campbell G, Mikolajczak M, McGill K, Mahad D, Fleetwood Walker S. A Targeted Mutation Disrupting Mitochondrial Complex IV Function in Primary Afferent Neurons Leads to Pain Hypersensitivity Through P2Y1 Receptor Activation. Mol Neurobiol. 2019;: pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates: - 1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).4-6. Anti-P2Y1 Receptor (extracellular) Antibody, preincubated with P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021).

image 2 :

Expression of P2RY1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.

image 3 :

Cell surface detection of P2RY1 in Jurkat (human acute T cell leukemia) living cells: - ___Unstained cells.___Cells stained +Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) (5 ?g/1x106cells).

product information

CAT :

APR-021

SKU :

APR-021-CF_0.2 ml

Product Name :

Anti-P2Y1 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47900

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR021

Homology :

Rat - identical; mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 1, P2RY1

Short Description :

A Rabbit Polyclonal Antibody to P2Y1 Receptor

Long Description :

Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples.

Negative Control :

BLP-PR021

Positive Control :

Synonyms :

P2Y purinoceptor 1, P2RY1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage