Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody | Alomone Labs APR-021-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody

catalog :

APR-021-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Hevia M, Castro P, Pinto K, Reyna Jeldes M, Rodr xed guez Tirado F, Robles Planells C, et al. Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors. Front Pharmacol. 2019;10:612 pubmed publisher
Odom M, Pak E, Brown D, Hannan J. Enhanced Electrical Field Stimulated Nitrergic and Purinergic Vasoreactivity in Distal vs Proximal Internal Pudendal Arteries. J Sex Med. 2017;14:1285-1296 pubmed publisher

image

image 1 :

Expression of P2RY1 in mouse brain - Immunohistochemical staining of mouse hippocampus using Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-021-AG), (1:80). A. P2RY1 staining (green) is detected in pyramidal cells (horizontal arrow) and in apical dendrites (vertical arrow). B. Nuclei staining using DAPI as the counterstain. C. Merged image of panels A and B.

image 2 :

Cell surface detection of P2RY1 in intact living Jurkat cells: Cell surface detection of P2RY1 in intact living Jurkat cells: Black line: Unstained cells. Green line: Cells + Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 antibody, (#APR-021-AG), (5 µg/5x105 cells.).

product information

CAT :

APR-021-AG

SKU :

APR-021-AG_50 mcl

Product Name :

Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47900

Applications :

FC IC IF IHC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR021

Homology :

Rat - identical; mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

Applications may also work in :

FC IC IF IHC LCI

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 1, P2RY1

Short Description :

A Rabbit Polyclonal Antibody to P2Y1 Receptor Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples. Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-021-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

P2Y purinoceptor 1, P2RY1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-021), and Direct Flow Cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:80

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage