Anti-P2Y12 Receptor (extracellular) Antibody | Alomone Labs APR-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y12 Receptor (extracellular) Antibody

catalog :

APR-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:100; fig 1b	Sellgren C, Sheridan S, Gracias J, Xuan D, Fu T, Perlis R. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry. 2017;22:170-177 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; tbl 1	Mildner A, Huang H, Radke J, Stenzel W, Priller J. P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Glia. 2017;65:375-387 pubmed publisher
	Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher
	Ryan S, Jordan Sciutto K, Anderson S. Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia. STAR Protoc. 2020;1:100190 pubmed publisher
	Woehrle T, Ledderose C, Rink J, Slubowski C, Junger W. Autocrine stimulation of P2Y1 receptors is part of the purinergic signaling mechanism that regulates T cell activation. Purinergic Signal. 2019;: pubmed publisher
	Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
	Jiang P, Xing F, Guo B, Yang J, Li Z, Wei W, et al. Nucleotide transmitters ATP and ADP mediate intercellular calcium wave communication via P2Y12/13 receptors among BV-2 microglia. PLoS ONE. 2017;12:e0183114 pubmed publisher
	Douvaras P, Sun B, Wang M, Kruglikov I, Lallos G, Zimmer M, et al. Directed Differentiation of Human Pluripotent Stem Cells to Microglia. Stem Cell Reports. 2017;8:1516-1524 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell line lysate: - 1-2. Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:200).3-4. Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).

image 2 :

Peptide CTAENTLFYVKES corresponding to amino acid residues 270-282 of human P2RY12 (AccessionQ9H244). 3rdextracellular loop.

image 3 :

Cell surface detection ofP2RY12 inlive intacthuman MEG-O1cells: - ___Unstained cells.___Cells +Anti-P2Y12 Receptor (extracellular)Antibody (#APR-020) (5-10 g/1x106cells).

product information

CAT :

APR-020

SKU :

APR-020-CF_0.2 ml

Product Name :

Anti-P2Y12 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

IC IF IFC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR020

Homology :

Rat, mouse - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3. The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Short Description :

A Rabbit Polyclonal Antibody to P2Y12 Receptor

Long Description :

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples.

Negative Control :

BLP-PR020

Positive Control :

Synonyms :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage