Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody | Alomone Labs APR-020-V product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody

catalog :

APR-020-V

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of P2RY12 by direct flow cytometry in live intact mouse BV-2 microglia cells:

image 2 :

Cell surface detection of P2RY12 by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:

image 3 :

Cell surface detection of P2RY12 by direct flow cytometry in live intact mouse SIM-A9 microglia cells :

product information

CAT :

APR-020-V

SKU :

APR-020-V_15 mcl

Product Name :

Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR020

Homology :

Rat, mouse - 12/13 amino acid residues identical.

Formulation :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3. The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 12, P2RY12, P2Y receptor

Short Description :

A Rabbit Polyclonal Antibody to P2Y12 Receptor conjugated to mFluor™ Violet 450 fluorophore

Long Description :

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples. Anti-P2Y12 Receptor (extracellular)-mFluor™ Violet 450 Antibody (#APR-020-V) is directly conjugated to mFluor™ Violet 450 fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. mFluor™ Violet 450 dye has a maximum absorption of 405 nm and maximum emission of 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.

Negative Control :

RIC-001-V

Synonyms :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Purity :

Affinity purified on immobilized antigen.

Negative control name :

Rabbit IgG Isotype Control-mFluor™ Violet 450

UNSPSC :

41116161

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-020), and direct flow cytometry (labeled antibody).

more info or order :

Alomone Labs product webpage