antibody

Anti-P2Y12 Receptor (extracellular)-PE Antibody

APR-020-PE

polyclonal

domestic rabbit

PE

NA

human, mouse, rat

APR-020-PE_15 mcl

Anti-P2Y12 Receptor (extracellular)-PE Antibody

Antibodies

Antibodies

Polyclonal

Q9H244

FC LCI

Human Rat Mouse

Rabbit

BLP-PR020

Rat, mouse - 12/13 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

3rd extracellular loop

R-Phycoerythrin (R-PE).

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

The P2Y receptors are a widely expressed group of G-protein coupled receptors (GPCRs). P2Y receptors are activated by nucleotides which are extracellular signaling molecules that are released from damaged cells or secreted via non-lytic mechanisms during inflammation, ischemia or hypoxia.P2Y12 receptor, like all P2Y receptors, is a 7-membrane spanning protein coupled to a G protein. Most of the seven transmembrane helices of P2YR12 are not perpendicular to the plane of the membrane but are tilted or kinked. The carboxy-terminal helix VIII is parallel to the membrane lipid bilayer. The receptor contains 342 amino acid residues and two potential N-linked glycosylation sites at its extra-cellular amino terminus, which may modulate its activity.ADP and some of its analogues stimulate P2YR12-mediated inhibition of adenylyl cyclase through activation of the Gαi2 G protein subtype, although effective coupling may also occur to Gαi1 G and Gαi3 G.P2YR12 is an important cofactor of platelet aggregation and secretion induced by several agonists including collagen, TXA2, thrombin and specific antibodies cross-linking FcγRIIa receptors. It also plays an important role in shear-induced platelet aggregation and in platelet thrombus formation and stabilization on collagen-coated surfaces or ruptured atherosclerotic plaques under flow conditions.Inherited abnormalities of P2YR12 include an autosomal recessive disorder causing severe receptor deficiency. Due to this abnormality, ADP, even at very high concentrations, does not induce full and irreversible platelet aggregation, nor does it inhibit the stimulation of platelet adenylyl cyclase causing excessive bleeding and additional coagulative dysfunction.

FC LCI

Alomone Labs

P2Y purinoceptor 12, P2RY12, P2Y receptor

A Rabbit Polyclonal Antibody to P2Y12 Conjugated to the Fluorescent Dye Phycoerythrin

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples. Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

RIC-001-PE

NA

P2Y purinoceptor 12, P2RY12, P2Y receptor

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

P2RY12

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

ICC FC

NA

Western blot analysis (unlabeled antibody, #APR-020), and direct flow cytometry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information