Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody | Alomone Labs APR-020-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody

catalog :

APR-020-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher
SHERIDAN S, Thanos J, De Guzman R, McCrea L, Horng J, Fu T, et al. Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure. Transl Psychiatry. 2021;11:179 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:400).3,4. Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody, preincubated with P2Y12 Receptor (extracellular) Blocking Peptide (#BLP-PR020).

product information

CAT :

APR-020-GP

SKU :

APR-020-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PR020

Homology :

Mouse - 12/13 amino acid residues identical; rat - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3. The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Short Description :

A Guinea Pig Polyclonal Antibody to P2Y12 Receptor

Long Description :

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP, formerly AGP-098) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot and live imaging applications. It and has been designed to recognize P2RY12 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PR020

Positive Control :

Synonyms :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage