Anti-P2Y14 Receptor (extracellular) Antibody | Alomone Labs APR-018 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y14 Receptor (extracellular) Antibody

catalog :

APR-018

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Patritti Cram J, Wu J, Coover R, Rizvi T, Chaney K, Ravindran R, et al. P2RY14 cAMP signaling regulates Schwann cell precursor self-renewal, proliferation, and nerve tumor initiation in a mouse model of neurofibromatosis. elife. 2022;11: pubmed publisher
Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
Li F, Li W, Li X, Li F, Zhang L, Wang B, et al. Geniposide attenuates inflammatory response by suppressing P2Y14 receptor and downstream ERK1/2 signaling pathway in oxygen and glucose deprivation-induced brain microvascular endothelial cells. J Ethnopharmacol. 2016;185:77-86 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 4), brain (lanes 2 and 5) and lung lysates (lanes 3 and 6): - 1-3. Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018), (1:200).4-6. Anti-P2Y14 Receptor (extracellular) Antibody, preincubated with P2Y14 Receptor (extracellular) Blocking Peptide (#BLP-PR018).

image 2 :

Expression of P2Y14 in rat pancreas. - Immunohistochemical staining of paraffin-embedded sections of rat pancreas with Anti-P2Y14 (extracellular) Antibody (#APR-018), (1:50), followed by SuperPicture HRP polymer. A. Staining in the rat pancreas showed P2Y14 immunoreactivity (brown) in endocrine cells of the Isles of Langerhans (arrows). Counterstain is Hematoxilin.

image 3 :

Expression of P2Y14 receptor in mouse kidney. - Immunohistochemical staining of mouse kidney sections using Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018). P2Y14 receptor staining (green) is detected in intercalated cells only of the cortex (A) and medulla (B). P2Y14 receptor co-localizes with V-ATPase B1 subunit (red staining) in intercalated cells but not in distal tubule cells. Pre-incubation of the control peptide antigen abolishes P2Y14 staining in both cortex (C) and medulla (D).Adapted from Azroyan A. et al. (2015) with kind permission of Dr. Breton S. of Center for Systems Biology Massachusetts General Hospital/Harvard Medical School Boston Massachusetts USA.

product information

CAT :

APR-018

SKU :

APR-018-CF_0.2 ml

Product Name :

Anti-P2Y14 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q15391

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR018

Homology :

Rat, mouse - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2RY14

Product Page - Scientific background :

P2Y14 belongs to the P2Y receptor family that is part of the G-protein coupled receptor superfamily. To date, eight functional mammalian P2Y receptors have been identified: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13 and P2Y14.The P2Y receptor family mediates the effects of extracellular nucleotides such as ATP, ADP, UTP, and in the case of P2Y14, UDP-glucose. UDP-glucose, as with the other nucleotides, can be released to the extracellular milieu as the result of cell damage or by regulated non-lytic mechanisms.P2Y14 is expressed in several tissues including the brain, lung and several cells of hematopoietic origin such as T lymphocytes and dendritic cells.The exact function of this receptor in the different tissues has not been clearly established but an involvement in chemotaxis, maturation of immature dendritic cells and neuroimmune regulation has been suggested.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor

Short Description :

A Rabbit Polyclonal Antibody to P2Y14 Receptor

Long Description :

Anti-P2Y14 (extracellular) Antibody (#APR-018) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus highly suited for detecting the receptor in living cells. It has been designed to recognize P2Y14 from human, rat, and mouse samples.

Negative Control :

BLP-PR018

Positive Control :

Synonyms :

P2Y purinoceptor 14, P2RY14, GPR105, UDP-glucose receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY14

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage