Anti-P2Y13 Receptor Antibody | Alomone Labs APR-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y13 Receptor Antibody

catalog :

APR-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Jairaman A, McQuade A, Granzotto A, Kang Y, Chadarevian J, GANDHI S, et al. TREM2 regulates purinergic receptor-mediated calcium signaling and motility in human iPSC-derived microglia. elife. 2022;11: pubmed publisher
Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
Kyrargyri V, Madry C, Rifat A, Arancibia Carcamo I, Jones S, Chan V, et al. P2Y13 receptors regulate microglial morphology, surveillance, and resting levels of interleukin 1β release. Glia. 2019;: pubmed publisher
Alves M, de Diego García L, Conte G, Jimenez Mateos E, D Orsi B, Sanz Rodriguez A, et al. Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y1 Receptor in Experimental Epilepsy. J Neurosci. 2019;39:5377-5392 pubmed publisher
Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
Jakovljevic M, Lavrnja I, Bozic I, Savic D, Bjelobaba I, Pekovic S, et al. Down-regulation of NTPDase2 and ADP-sensitive P2 Purinoceptors Correlate with Severity of Symptoms during Experimental Autoimmune Encephalomyelitis. Front Cell Neurosci. 2017;11:333 pubmed publisher
Guarracino J, Cinalli A, Fernandez V, Roquel L, Losavio A. P2Y13 receptors mediate presynaptic inhibition of acetylcholine release induced by adenine nucleotides at the mouse neuromuscular junction. Neuroscience. 2016;326:31-44 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-P2Y13 Receptor Antibody (APR-017), (1:200).2. Anti-P2Y13 Receptor Antibody, preincubated with P2Y13 Receptor Blocking Peptide (#BLP-PR017).

image 2 :

Expression of P2RY13 in rat testis - Immunohistochemical staining of paraffin embedded rat epidydimus section using Anti-P2Y13 Receptor Antibody (#APR-017), (1:50). Strong and high specific staining of the epithelial cells is shown. Universal Immuno-alkaline-phosphatase kit followed by New Fuchsin Substrate was used for color reaction (red). Hematoxilin is used as the counterstain.

image 3 :

Peptide DRFLKIIRPLRNIFLK(C) corresponding to amino acid residues 119-134 of human P2RY13 (AccessionQ9BPV8). 2ndintracellular loop.

product information

CAT :

APR-017

SKU :

APR-017-CF_0.2 ml

Product Name :

Anti-P2Y13 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9BPV8

Applications :

IC IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR017

Homology :

Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2RY13

Product Page - Scientific background :

The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions.A new member of this family was recently identified, the P2Y13 receptor. The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gai class of G-proteins. The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1,2 Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.3P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.4

Applications may also work in :

IC IF IFC IHC WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 13, P2RY13, GPCR1, GPR86, GPR94

Short Description :

A Rabbit Polyclonal Antibody to P2Y13 Receptor

Long Description :

Anti-P2Y13 Receptor Antibody (#APR-017) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY13 in human, rat, and mouse samples.

Negative Control :

BLP-PR017

Positive Control :

Synonyms :

P2Y purinoceptor 13, P2RY13, GPCR1, GPR86, GPR94

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY13

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage