Anti-P2X3 Receptor Antibody | Alomone Labs APR-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X3 Receptor Antibody

catalog :

APR-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:500; loading ...; fig 6a, 4b	Larson E, Vandenbeuch A, Anderson C, Kinnamon S. Function, Innervation, and Neurotransmitter Signaling in Mice Lacking Type-II Taste Cells. Eneuro. 2020;7: pubmed publisher
western blot; mouse; 1:1000; fig 2	Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
	Wang Z, Jung J, Inbar T, Rangoussis K, Faaborg Andersen C, Coate T. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. Eneuro. 2020;7: pubmed publisher
	Wilson C, Vandenbeuch A, Kinnamon S. Physiological and Behavioral Responses to Optogenetic Stimulation of PKD2L1+ Type III Taste Cells. Eneuro. 2019;6: pubmed publisher
	Carluccio M, Zuccarini M, Ziberi S, Giuliani P, Morabito C, Mariggio M, et al. Involvement of P2X7 Receptors in the Osteogenic Differentiation of Mesenchymal Stromal/Stem Cells Derived from Human Subcutaneous Adipose Tissue. Stem Cell Rev. 2019;: pubmed publisher
	Lu V, Rievaj J, O Flaherty E, Smith C, Pais R, Pattison L, et al. Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons. Nat Commun. 2019;10:1029 pubmed publisher
	Arribas Blázquez M, Olivos Oré L, Barahona M, Sánchez de la Muela M, Solar V, Jiménez E, et al. Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain. Int J Mol Sci. 2019;20: pubmed publisher
	Kumari A, Yokota Y, Li L, Bradley R, Mistretta C. Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib. Sci Rep. 2018;8:16150 pubmed publisher
	Romanov R, Lasher R, High B, Savidge L, Lawson A, Rogachevskaja O, et al. Chemical synapses without synaptic vesicles: Purinergic neurotransmission through a CALHM1 channel-mitochondrial signaling complex. Sci Signal. 2018;11: pubmed publisher
	Stephan G, Huang L, Tang Y, Vilotti S, Fabbretti E, Yu Y, et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018;9:1354 pubmed publisher
	Mah W, Lee S, Lee J, Bae J, Ju J, Lee C, et al. A role for the purinergic receptor P2X3 in astrocytes in the mechanism of craniofacial neuropathic pain. Sci Rep. 2017;7:13627 pubmed publisher
	Coddou C, Sandoval R, Castro P, Lazcano P, Hevia M, Rokic M, et al. Cyclin-dependent kinase 5 modulates the P2X2a receptor channel gating through phosphorylation of C-terminal threonine 372. Pain. 2017;158:2155-2168 pubmed publisher

image

image 1 :

Western blot analysis of rat DRG lysates: - 1. Anti-P2X3 Receptor Antibody (APR-016), (1:200).2. Anti-P2X3 Receptor Antibody, preincubated with P2X3 Receptor Blocking Peptide (#BLP-PR016).

image 2 :

Expression of P2X3 Receptor in rat DRG neurons - Immunohistochemical staining of rat dorsal root ganglion (DRG) neurons with Anti-P2X3 Receptor Antibody (#APR-016), (A). Cells within the DRG were stained (see solid line frame enlarged (B) as well as fibers and the area of entry of dorsal root into spinal cord (see dashed line frame enlarged (C). DAPI is used as the counterstain (in B and C).

image 3 :

Western blot analysisof rat DRG lysates: - 1.Anti-P2X3 ReceptorAntibody (APR-016) (1:200).2.Anti-P2X3 Receptor Antibody preincubated with the negative control antigen.

product information

CAT :

APR-016

SKU :

APR-016-CF_0.2 ml

Product Name :

Anti-P2X3 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49654

Applications :

IC IF IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR016

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor

Product Page - Scientific background :

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Applications may also work in :

IC IF IFC IHC WB

Supplier :

Alomone Labs

Target :

P2RX3, P2X purinoceptor 3, ATP Receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X3 Receptor

Long Description :

Anti-P2X3 Receptor Antibody (#APR-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR016

Positive Control :

Synonyms :

P2RX3, P2X purinoceptor 3, ATP Receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage