Guinea Pig Anti-P2X3 Antibody | Alomone Labs APR-016-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea Pig Anti-P2X3 Antibody

catalog :

APR-016-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wang Z, Jung J, Inbar T, Rangoussis K, Faaborg Andersen C, Coate T. The Purinergic Receptor P2rx3 is Required for Spiral Ganglion Neuron Branch Refinement during Development. Eneuro. 2020;7: pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysates: - 1. Guinea Pig Anti-P2X3 Antibody (#APR-016-GP), (1:400). 2. Guinea Pig Anti-P2X3 Antibody, preincubated with P2X3 Blocking Peptide (BLP-PR016).

image 2 :

Expression of P2X3 in rat dorsal root ganglion. - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) using Guinea pig Anti-P2X3 Antibody (#APR-016-GP), (1:200), followed by goat anti-guinea pig-AlexaFluor-594. A. P2X3 immunoreactivity (red) appears in neuronal profiles (arrows). B. Pre-incubation of the antibody with P2X3 Blocking Peptide (BLP-PR016), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APR-016-GP

SKU :

APR-016-GP-CF_0.2 ml

Product Name :

Guinea Pig Anti-P2X3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49654

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PR016

Homology :

Mouse - identical; human - 13 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor

Product Page - Scientific background :

The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

P2RX3, P2X purinoceptor 3, ATP Receptor

Short Description :

A Guinea Pig Polyclonal Antibody to P2X3 Receptor

Long Description :

Guinea Pig Anti-P2X3 Receptor Antibody (#APR-016-GP, formerly AGP-284) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, and immunohistochemistry applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2X3 Antibody (#APR-016) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Negative Control :

BLP-PR016

Positive Control :

Synonyms :

P2RX3, P2X purinoceptor 3, ATP Receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage