Anti-Human P2Y11 Receptor Antibody | Alomone Labs APR-015 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human P2Y11 Receptor Antibody

catalog :

APR-015

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
Bátori R, Kumar S, Bordán Z, Cherian Shaw M, Kovacs Kasa A, MacDonald J, et al. Differential mechanisms of adenosine- and ATPγS-induced microvascular endothelial barrier strengthening. J Cell Physiol. 2019;234:5863-5879 pubmed publisher
Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher

image

image 1 :

Western blot analysis of human platelet lysates: - 1. Anti-Human P2Y11 Receptor Antibody (#APR-015), (1:200).2. Anti-Human P2Y11 Receptor Antibody, preincubated with Human P2Y11 Receptor Blocking Peptide (#BLP-PR015).

image 2 :

Peptide (C)NATAAPKPSEPQSRELS corresponding to amino acid residues 357-373 of human P2RY11 (AccessionQ96G91). Intracellular C-terminus.

image 3 :

Western blot analysis of human platelet lysates: - 1.Anti-Human P2Y11 Receptor Antibody (#APR-015) (1:200).2. Anti-Human P2Y11 Receptor Antibody preincubated with the negative control antigen.

product information

CAT :

APR-015

SKU :

APR-015-CF_0.2 ml

Product Name :

Anti-Human P2Y11 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q96G91

Applications :

IC IF IFC IP WB

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-PR015

Homology :

Human only Not recommended for use with rat and mouse samples

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2RY11

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly expressed in the spleen and to a much lower extent in platelets.Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4

Applications may also work in :

IC IF IFC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 11, P2RY11

Short Description :

A Rabbit Polyclonal Antibody to P2Y11 Receptor

Long Description :

Anti-Human P2Y11 Receptor Antibody (#APR-015) is a highly specific antibody directed against an epitope of the human P2RY11. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize P2RY11 from human samples.

Negative Control :

BLP-PR015

Positive Control :

Synonyms :

P2Y purinoceptor 11, P2RY11

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY11

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage