Anti-P2Y12 Receptor Antibody | Alomone Labs APR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y12 Receptor Antibody

catalog :

APR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2j	Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1	Walker D, Tang T, Mendsaikhan A, Tooyama I, Serrano G, Sue L, et al. Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer's Disease Brains. Int J Mol Sci. 2020;21: pubmed publisher
	Quek H, Cuní López C, Stewart R, Colletti T, Notaro A, Nguyen T, et al. ALS monocyte-derived microglia-like cells reveal cytoplasmic TDP-43 accumulation, DNA damage, and cell-specific impairment of phagocytosis associated with disease progression. J Neuroinflammation. 2022;19:58 pubmed publisher
	Defaye M, Abdullah N, Iftinca M, Hassan A, Agosti F, Zhang Z, et al. Gut-innervating TRPV1+ Neurons Drive Chronic Visceral Pain via Microglial P2Y12 Receptor. Cell Mol Gastroenterol Hepatol. 2022;13:977-999 pubmed publisher
	Ronzano R, Roux T, Thetiot M, Aigrot M, Richard L, Lejeune F, et al. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination. Nat Commun. 2021;12:5219 pubmed publisher
	Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher
	Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
	Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
	Alves M, de Diego García L, Conte G, Jimenez Mateos E, D Orsi B, Sanz Rodriguez A, et al. Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y1 Receptor in Experimental Epilepsy. J Neurosci. 2019;39:5377-5392 pubmed publisher
	Zou L, Han X, Liu S, Gong Y, Wu B, Yi Z, et al. Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia. Front Physiol. 2018;9:928 pubmed publisher
	Yi Z, Xie L, Zhou C, Yuan H, Ouyang S, Fang Z, et al. P2Y12 receptor upregulation in satellite glial cells is involved in neuropathic pain induced by HIV glycoprotein 120 and 2',3'-dideoxycytidine. Purinergic Signal. 2018;14:47-58 pubmed publisher
	Koessler J, Schwarz M, Weber K, Etzel J, Koessler A, Böeck M, et al. The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions. PLoS ONE. 2017;12:e0188193 pubmed publisher
	Kamiyama M, Shirai T, Tamura S, Suzuki Inoue K, Ehata S, Takahashi K, et al. ASK1 facilitates tumor metastasis through phosphorylation of an ADP receptor P2Y12 in platelets. Cell Death Differ. 2017;24:2066-2076 pubmed publisher
	Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (1 and 2) and human platelets (3 and 4): - 1,3. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200). 2,4. Anti-P2Y12 Receptor Antibody, preincubated with P2Y12 Receptor Blocking Peptide (#BLP-PR012).

image 2 :

Expression of P2RY12 in mouse brain - Immunohistochemical staining of mouse brain sections using Anti-P2Y12 Receptor Antibody (#APR-012), (red). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.

product information

CAT :

APR-012

SKU :

APR-012-CF_0.2 ml

Product Name :

Anti-P2Y12 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR012

Homology :

Rat, mouse - 16/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 2nd cytoplasmic loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Short Description :

A Rabbit Polyclonal Antibody to P2Y12 Receptor

Long Description :

Anti-P2Y12 Receptor Antibody (#APR-012) is a highly specific antibody directed against an intracellular epitope of the human P2RY12. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize P2RY12 from human, rat, and mouse samples.

Negative Control :

BLP-PR012

Positive Control :

Synonyms :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage