Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody | Alomone Labs APR-012-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody

catalog :

APR-012-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher

image

image 1 :

Expression of P2RY12 in rat striatum - Immunohistochemical staining of rat striatum using Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR). P2Y12 (red) appears in patches (white arrows) of myelinated axons. DAPI is used as the counterstain (blue).

product information

CAT :

APR-012-AR

SKU :

APR-012-AR_50 mcl

Product Name :

Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR012

Homology :

Rat, mouse - 16/18 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, 2nd cytoplasmic loop

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1,P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Short Description :

A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-P2Y12 Receptor Antibody (#APR-012) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize P2RY12 from human, rat, and mouse samples. Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-PR012

Positive Control :

Synonyms :

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY12

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-012), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage