Anti-P2Y6 Receptor Antibody | Alomone Labs APR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y6 Receptor Antibody

catalog :

APR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Shin S, Jeong J, Kim J, Sohn K, Yoon S, Kim J. 1-Palmitoyl-2-Linoleoyl-3-Acetyl-rac-Glycerol (PLAG) Mitigates Monosodium Urate (MSU)-Induced Acute Gouty Inflammation in BALB/c Mice. Front Immunol. 2020;11:710 pubmed publisher
Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
Mahdi A, Jiao T, Tratsiakovich Y, Yang J, Ostenson C, Pernow J, et al. Altered Purinergic Receptor Sensitivity in Type 2 Diabetes-Associated Endothelial Dysfunction and Up₄A-Mediated Vascular Contraction. Int J Mol Sci. 2018;19: pubmed publisher
Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
Li xf1 xe1 n Rico A, Ochoa Cortes F, Zuleta Alarcon A, Alhaj M, Tili E, Enneking J, et al. UTP - Gated Signaling Pathways of 5-HT Release from BON Cells as a Model of Human Enterochromaffin Cells. Front Pharmacol. 2017;8:429 pubmed publisher
Lommen J, Stahr A, Ingenwerth M, Ali A, Von Gall C. Time-of-day-dependent expression of purinergic receptors in mouse suprachiasmatic nucleus. Cell Tissue Res. 2017;369:579-590 pubmed publisher
Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-P2Y6 Receptor Antibody (#APR-011), (1:200).2. Anti-P2Y6 Receptor Antibody, preincubated with P2Y6 Receptor Blocking Peptide (#BLP-PR011).

image 2 :

Expression of P2Y6 in rat cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y6 Receptor Antibody (#APR-011), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. P2Y6 immunoreactivity (red) appears in cortical neurons (horizontal arrows) and astrocytes (vertical arrows). B. Pre-incubation of the antibody with P2Y6 Receptor Blocking Peptide (BLP-PR011), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysis of mouse brain lysate: - 1.Anti-CRTH2/GPR44 (extracellular) Antibody(#APR-062) (1:400).2. Anti-CRTH2/GPR44 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APR-011

SKU :

APR-011-CF_0.2 ml

Product Name :

Anti-P2Y6 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63371

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR011

Homology :

Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor

Product Page - Scientific background :

The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily.1,3P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels.1,2P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries.4

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 6, P2RY6

Short Description :

A Rabbit Polyclonal Antibody to P2Y6 Receptor

Long Description :

Anti-P2Y6 Receptor Antibody (#APR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY6 from rat, mouse, and human samples.

Negative Control :

BLP-PR011

Positive Control :

Synonyms :

P2Y purinoceptor 6, P2RY6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage