Anti-P2Y2 Receptor Antibody | Alomone Labs APR-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y2 Receptor Antibody

catalog :

APR-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; fig 2	Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
	Tomas Bort E, Joseph M, Wang Q, Carter E, Roth N, Gibson J, et al. Purinergic GPCR-integrin interactions drive pancreatic cancer cell invasion. elife. 2023;12: pubmed publisher
	Donati V, Peres C, Nardin C, Scavizzi F, Raspa M, Ciubotaru C, et al. Calcium Signaling in the Photodamaged Skin: In Vivo Experiments and Mathematical Modeling. Function (Oxf). 2022;3:zqab064 pubmed publisher
	Nardin C, Peres C, Putti S, Orsini T, Colussi C, Mazzarda F, et al. Connexin Hemichannel Activation by S-Nitrosoglutathione Synergizes Strongly with Photodynamic Therapy Potentiating Anti-Tumor Bystander Killing. Cancers (Basel). 2021;13: pubmed publisher
	Daneva Z, Ottolini M, Chen Y, Klimentova E, Kuppusamy M, Shah S, et al. Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice. elife. 2021;10: pubmed publisher
	Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
	Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
	Hevia M, Castro P, Pinto K, Reyna Jeldes M, Rodr xed guez Tirado F, Robles Planells C, et al. Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors. Front Pharmacol. 2019;10:612 pubmed publisher
	Solís Chagoyán H, Flores Soto E, Valdés Tovar M, Cercós M, Calixto E, Montaño L, et al. Purinergic Signaling Pathway in Human Olfactory Neuronal Precursor Cells. Stem Cells Int. 2019;2019:2728786 pubmed publisher
	Alves M, de Diego García L, Conte G, Jimenez Mateos E, D Orsi B, Sanz Rodriguez A, et al. Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y1 Receptor in Experimental Epilepsy. J Neurosci. 2019;39:5377-5392 pubmed publisher
	Kristiansen S, Skovsted G, Berchtold L, Radziwon Balicka A, Dreisig K, Edvinsson L, et al. Role of pannexin and adenosine triphosphate (ATP) following myocardial ischemia/reperfusion. Scand Cardiovasc J. 2018;52:340-343 pubmed publisher
	Palygin O, Ilatovskaya D, Levchenko V, Klemens C, Dissanayake L, Williams A, et al. Characterization of purinergic receptor expression in ARPKD cystic epithelia. Purinergic Signal. 2018;14:485-497 pubmed publisher
	Henriquez M, Fonseca M, Perez Zoghbi J. Purinergic receptor stimulation induces calcium oscillations and smooth muscle contraction in small pulmonary veins. J Physiol. 2018;596:2491-2506 pubmed publisher
	Paniagua Herranz L, Gil Redondo J, Queipo M, Gonzalez Ramos S, Bosca L, Pérez Sen R, et al. Prostaglandin E2 Impairs P2Y2/P2Y4 Receptor Signaling in Cerebellar Astrocytes via EP3 Receptors. Front Pharmacol. 2017;8:937 pubmed publisher
	Wu D, Lee S, Luo J, Xia H, Gushchina S, Richardson P, et al. Intraneural Injection of ATP Stimulates Regeneration of Primary Sensory Axons in the Spinal Cord. J Neurosci. 2018;38:1351-1365 pubmed publisher
	Fonseca B, Martínez Águila A, Pérez de Lara M, Miras Portugal M, Gomez Villafuertes R, Pintor J. Changes in P2Y Purinergic Receptor Expression in the Ciliary Body in a Murine Model of Glaucoma. Front Pharmacol. 2017;8:719 pubmed publisher
	Ellegaard M, Agca C, Petersen S, Agrawal A, Kruse L, Wang N, et al. Bone turnover is altered in transgenic rats overexpressing the P2Y2 purinergic receptor. Purinergic Signal. 2017;13:545-557 pubmed publisher
	Li xf1 xe1 n Rico A, Ochoa Cortes F, Zuleta Alarcon A, Alhaj M, Tili E, Enneking J, et al. UTP - Gated Signaling Pathways of 5-HT Release from BON Cells as a Model of Human Enterochromaffin Cells. Front Pharmacol. 2017;8:429 pubmed publisher
	Robles Martínez L, Garay E, Martel Gallegos M, Cisneros Mejorado A, Perez Montiel D, Lara A, et al. Kca3.1 Activation Via P2y2 Purinergic Receptors Promotes Human Ovarian Cancer Cell (Skov-3) Migration. Sci Rep. 2017;7:4340 pubmed publisher
	Shi Y, Qin W, Nie F, Wen H, Lu K, Cui J. Ulinastatin attenuates neuropathic pain via the ATP/P2Y2 receptor pathway in rat models. Gene. 2017;627:263-270 pubmed publisher
	Mart xed nez Ram xed rez A, D xed az Mu xf1 oz M, Battastini A, Campos Contreras A, Olvera A, Bergamin L, et al. Cellular Migration Ability Is Modulated by Extracellular Purines in Ovarian Carcinoma SKOV-3 Cells. J Cell Biochem. 2017;118:4468-4478 pubmed publisher
	Hawkins V, Takakura A, Trinh A, Malheiros Lima M, Cleary C, Wenker I, et al. Purinergic regulation of vascular tone in the retrotrapezoid nucleus is specialized to support the drive to breathe. elife. 2017;6: pubmed publisher
	Wu L, Oshima T, Fukui H, Watari J, Miwa H. Adenosine triphosphate induces P2Y2 activation and interleukin-8 release in human esophageal epithelial cells. J Gastroenterol Hepatol. 2017;32:1341-1347 pubmed publisher
	Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
	Sathanoori R, Bryl Gorecka P, Muller C, Erb L, Weisman G, Olde B, et al. P2Y2 receptor modulates shear stress-induced cell alignment and actin stress fibers in human umbilical vein endothelial cells. Cell Mol Life Sci. 2017;74:731-746 pubmed publisher
	Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher
	Cui J, Xu M, Liu E, Dong T, Lin H, Tsim K, et al. Expression of globular form acetylcholinesterase is not altered in P2Y1R knock-out mouse brain. Chem Biol Interact. 2016;259:291-294 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1, 2. Anti-P2Y2 Receptor Antibody (#APR-010), (1:200). 3, 4. Anti-P2Y2 Receptor Antibody, preincubated with P2Y2 Receptor Blocking Peptide (#BLP-PR010).

image 2 :

Peptide (C)KPAY GTTGL PRAKR KSVR corresponding to residues 227-244 of rat P2RY2 (AccessionP41232). 3rdintracellular loop (i3) between TM5 and TM6 domains.

image 3 :

Western blot analysisof rat brain (lanes 1 3)and lung (lanes 2 4) membranes: - 12.Anti-P2Y2 ReceptorAntibody (#APR-010) (1:200).34. Anti-P2Y2 Receptor Antibody preincubated with the control peptide antigen.

product information

CAT :

APR-010

SKU :

APR-010-CF_0.2 ml

Product Name :

Anti-P2Y2 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P41232

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR010

Homology :

Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop (i3) between TM5 and TM6 domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14 .2-4The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway.5 It has become a leading candidate for the treatment of cystic fibrosis. Activation of P2Y2 receptor increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 2, P2RY2, P2U1

Short Description :

A Rabbit Polyclonal Antibody to P2Y2 Receptor

Long Description :

Anti-P2Y2 Receptor Antibody (#APR-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY2 in rat, mouse, and human samples.

Negative Control :

BLP-PR010

Positive Control :

Synonyms :

P2Y purinoceptor 2, P2RY2, P2U1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage