Anti-P2Y1 Receptor Antibody | Alomone Labs APR-009 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y1 Receptor Antibody

catalog :

APR-009

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Alomone Labs product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2i	Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 1c immunohistochemistry; human; 1:100; loading ...; fig 1a	Reichenbach N, Delekate A, Breithausen B, Keppler K, Poll S, Schulte T, et al. P2Y1 receptor blockade normalizes network dysfunction and cognition in an Alzheimer's disease model. J Exp Med. 2018;215:1649-1663 pubmed publisher
western blot; mouse; 1:1000; fig 2	Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
	Nardin C, Peres C, Putti S, Orsini T, Colussi C, Mazzarda F, et al. Connexin Hemichannel Activation by S-Nitrosoglutathione Synergizes Strongly with Photodynamic Therapy Potentiating Anti-Tumor Bystander Killing. Cancers (Basel). 2021;13: pubmed publisher
	Daneva Z, Ottolini M, Chen Y, Klimentova E, Kuppusamy M, Shah S, et al. Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice. elife. 2021;10: pubmed publisher
	Hamada K, Shinozaki Y, Namekata K, Matsumoto M, Ohno N, Segawa T, et al. Loss of P2Y1 receptors triggers glaucoma-like pathology in mice. Br J Pharmacol. 2021;178:4552-4571 pubmed publisher
	Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
	Hevia M, Castro P, Pinto K, Reyna Jeldes M, Rodr xed guez Tirado F, Robles Planells C, et al. Differential Effects of Purinergic Signaling in Gastric Cancer-Derived Cells Through P2Y and P2X Receptors. Front Pharmacol. 2019;10:612 pubmed publisher
	Blanke E, Stella S, Ruiz Velasco V, Holmes G. Purinergic receptor expression and function in rat vagal sensory neurons innervating the stomach. Neurosci Lett. 2019;706:182-188 pubmed publisher
	Alves M, de Diego García L, Conte G, Jimenez Mateos E, D Orsi B, Sanz Rodriguez A, et al. Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y1 Receptor in Experimental Epilepsy. J Neurosci. 2019;39:5377-5392 pubmed publisher
	Fukumoto Y, Tanaka K, Parajuli B, Shibata K, Yoshioka H, Kanemaru K, et al. Neuroprotective effects of microglial P2Y1 receptors against ischemic neuronal injury. J Cereb Blood Flow Metab. 2019;39:2144-2156 pubmed publisher
	Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
	Bátori R, Kumar S, Bordán Z, Cherian Shaw M, Kovacs Kasa A, MacDonald J, et al. Differential mechanisms of adenosine- and ATPγS-induced microvascular endothelial barrier strengthening. J Cell Physiol. 2019;234:5863-5879 pubmed publisher
	Koessler J, Schwarz M, Weber K, Etzel J, Koessler A, Böeck M, et al. The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions. PLoS ONE. 2017;12:e0188193 pubmed publisher
	Odom M, Pak E, Brown D, Hannan J. Enhanced Electrical Field Stimulated Nitrergic and Purinergic Vasoreactivity in Distal vs Proximal Internal Pudendal Arteries. J Sex Med. 2017;14:1285-1296 pubmed publisher
	Lu P, Hudgins R, Liu X, Ford Z, Hofmann M, Queme L, et al. Upregulation of P2Y1 in neonatal nociceptors regulates heat and mechanical sensitization during cutaneous inflammation. Mol Pain. 2017;13:1744806917730255 pubmed publisher
	Caputi V, Marsilio I, Cerantola S, Roozfarakh M, Lante I, Galuppini F, et al. Toll-Like Receptor 4 Modulates Small Intestine Neuromuscular Function through Nitrergic and Purinergic Pathways. Front Pharmacol. 2017;8:350 pubmed publisher
	Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
	Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher
	Cui J, Xu M, Liu E, Dong T, Lin H, Tsim K, et al. Expression of globular form acetylcholinesterase is not altered in P2Y1R knock-out mouse brain. Chem Biol Interact. 2016;259:291-294 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and human platelets lysate (lanes 2 and 4): - 1,2. Anti-P2Y1 Receptor Antibody (#APR-009), (1:200).3,4. Anti-P2Y1 Receptor Antibody, preincubated with P2Y1 Receptor Blocking Peptide (#BLP-PR009).

image 2 :

Expression of P2RY1 in rat thalamus - Immunohistochemical staining of fasciculi in rat thalamus using Anti-P2Y1 Receptor Antibody (#APR-009). Specific staining is black. Cresyl violet is used as the counterstain.

image 3 :

Peptide (C)RAHSVTTLRQLASR corresponding to amino acid residues 270-283 of rat PTGDR2 (AccessionQ6XKD3). 3rdextracellular loop.

product information

CAT :

APR-009

SKU :

APR-009-CF_0.2 ml

Product Name :

Anti-P2Y1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49651

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR009

Homology :

Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop (i3) between TM5 and TM6 domains.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1.

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 1, P2RY1

Short Description :

A Rabbit Polyclonal Antibody to P2Y1 Receptor

Long Description :

Anti-P2Y1 Receptor Antibody (#APR-009) is a highly specific antibody directed against an epitope of the human P2RY1. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2RY1 from human, rat, and mouse samples.

Negative Control :

BLP-PR009

Positive Control :

Synonyms :

P2Y purinoceptor 1, P2RY1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage