Anti-P2X7 Receptor (extracellular) Antibody | Alomone Labs APR-008 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X7 Receptor (extracellular) Antibody

catalog :

APR-008

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, proximity ligation assay

more info or order :

Alomone Labs product webpage

citations: 26

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:200; fig 3a	Campagno K, Lu W, Jassim A, Albalawi F, Cenaj A, Tso H, et al. Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressure. J Neuroinflammation. 2021;18:217 pubmed publisher
proximity ligation assay; mouse; loading ...; fig 4a	Rhodes G, Segars K, Lee Y, Hutcheon A, Rich C, Trinkaus Randall V. Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium. Anal Cell Pathol (Amst). 2021;2021:4793338 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 6c	Wang A, Wong V, Lee P, Bui B, Dudczig S, Vessey K, et al. Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout. Sci Rep. 2021;11:4184 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 1	Jager S, Vaegter C. Avoiding experimental bias by systematic antibody validation. Neural Regen Res. 2016;11:1079-80 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200; fig 4b	Giannuzzo A, Saccomano M, Napp J, Ellegaard M, Alves F, Novak I. Targeting of the P2X7 receptor in pancreatic cancer and stellate cells. Int J Cancer. 2016;139:2540-52 pubmed publisher
flow cytometry; mouse; fig 2 immunocytochemistry; mouse; fig 2 western blot; mouse; fig 2	Karmakar M, Katsnelson M, Dubyak G, Pearlman E. Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1Î² secretion in response to ATP. Nat Commun. 2016;7:10555 pubmed publisher
	Lee D, Kim J. Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus. J Neuroinflammation. 2021;18:14 pubmed publisher
	Pegoraro A, Orioli E, De Marchi E, Salvestrini V, Milani A, Di Virgilio F, et al. Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression. Cell Death Dis. 2020;11:876 pubmed publisher
	Benzaquen J, Dit Hreich S, Heeke S, Juhel T, Lalvee S, Bauwens S, et al. P2RX7B is a new theranostic marker for lung adenocarcinoma patients. Theranostics. 2020;10:10849-10860 pubmed publisher
	Qin J, Zhang X, Tan B, Zhang S, Yin C, Xue Q, et al. Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer. Cancer Immunol Res. 2020;8:1426-1439 pubmed publisher
	Brisson L, Chadet S, Lopez Charcas O, Jelassi B, Ternant D, Chamouton J, et al. P2X7 Receptor Promotes Mouse Mammary Cancer Cell Invasiveness and Tumour Progression, and Is a Target for Anticancer Treatment. Cancers (Basel). 2020;12: pubmed publisher
	Skarratt K, Gu B, Lovelace M, Milligan C, Stokes L, Glover R, et al. A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function. FASEB J. 2020;34:3884-3901 pubmed publisher
	Feehan A, Zadina J. Morphine immunomodulation prolongs inflammatory and postoperative pain while the novel analgesic ZH853 accelerates recovery and protects against latent sensitization. J Neuroinflammation. 2019;16:100 pubmed publisher
	Carluccio M, Zuccarini M, Ziberi S, Giuliani P, Morabito C, Mariggio M, et al. Involvement of P2X7 Receptors in the Osteogenic Differentiation of Mesenchymal Stromal/Stem Cells Derived from Human Subcutaneous Adipose Tissue. Stem Cell Rev. 2019;: pubmed publisher
	Reigada D, Calder xf3 n Garc xed a A, Soto Catal xe1 n M, Nieto D xed az M, Mu xf1 oz Galdeano T, Del xc1 guila x, et al. MicroRNA-135a-5p reduces P2X7 -dependent rise in intracellular calcium and protects against excitotoxicity. J Neurochem. 2019;151:116-130 pubmed publisher
	Palygin O, Ilatovskaya D, Levchenko V, Klemens C, Dissanayake L, Williams A, et al. Characterization of purinergic receptor expression in ARPKD cystic epithelia. Purinergic Signal. 2018;14:485-497 pubmed publisher
	Kaczmarek Hájek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, et al. Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody. elife. 2018;7: pubmed publisher
	D Addio F, Vergani A, Potena L, Maestroni A, Usuelli V, Ben Nasr M, et al. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes. J Clin Invest. 2018;128:3490-3503 pubmed publisher
	Komalavilas P, Luo W, Guth C, Jolayemi O, Bartelson R, Cheung Flynn J, et al. Vascular surgical stretch injury leads to activation of P2X7 receptors and impaired endothelial function. PLoS ONE. 2017;12:e0188069 pubmed publisher
	Green J, Souilhol C, Xanthis I, Martinez Campesino L, Bowden N, Evans P, et al. Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling. Cardiovasc Res. 2017;: pubmed publisher
	Leduc Pessah H, Weilinger N, Fan C, Burma N, Thompson R, Trang T. Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance. J Neurosci. 2017;37:10154-10172 pubmed publisher
	Albalawi F, Lu W, Beckel J, Lim J, McCaughey S, Mitchell C. The P2X7 Receptor Primes IL-1β and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain. Front Cell Neurosci. 2017;11:227 pubmed publisher
	Bartlett R, Sluyter V, Watson D, Sluyter R, Yerbury J. P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice. Peerj. 2017;5:e3064 pubmed publisher
	Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
	Migita K, Ozaki T, Shimoyama S, Yamada J, Nikaido Y, Furukawa T, et al. HSP90 Regulation of P2X7 Receptor Function Requires an Intact Cytoplasmic C-Terminus. Mol Pharmacol. 2016;90:116-26 pubmed publisher
	Elkhal A, Rodriguez Cetina Biefer H, Heinbokel T, Uehara H, Quante M, Seyda M, et al. NAD(+) regulates Treg cell fate and promotes allograft survival via a systemic IL-10 production that is CD4(+) CD25(+) Foxp3(+) T cells independent. Sci Rep. 2016;6:22325 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 5) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 6) and Mouse WEHI-231 B cell lymphoma (lanes 3 and 7) and human HL-60 promyelocytic leukemia cell line (lanes 4 and 8): - 1-4. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:200). 5-8. Anti-P2X7 Receptor (extracellular) Antibody, preincubated with P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008).

image 2 :

Expression of P2RX7 in rat pancreas - Immunohistochemical staining of rat pancreas frozen section using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.

product information

CAT :

APR-008

SKU :

APR-008-CF_0.2 ml

Product Name :

Anti-P2X7 Receptor (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR008

Homology :

Human, rat - identical; bovine - 14/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Product Page - Scientific background :

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X7 Receptor

Long Description :

Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.

Negative Control :

BLP-PR008

Positive Control :

Synonyms :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage