Anti-P2X7 Receptor (extracellular)-PE Antibody | Alomone Labs APR-008-PE product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X7 Receptor (extracellular)-PE Antibody

catalog :

APR-008-PE

clonality :

polyclonal

host :

domestic rabbit

conjugate :

clone name :

reactivity :

human, mouse

application :

flow cytometry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Narendran S, Pereira F, Yerramothu P, Apicella I, Wang S, Ambati K, et al. Nucleoside reverse transcriptase inhibitors and Kamuvudines inhibit amyloid-β induced retinal pigmented epithelium degeneration. Signal Transduct Target Ther. 2021;6:149 pubmed publisher
Benzaquen J, Dit Hreich S, Heeke S, Juhel T, Lalvee S, Bauwens S, et al. P2RX7B is a new theranostic marker for lung adenocarcinoma patients. Theranostics. 2020;10:10849-10860 pubmed publisher

image

image 1 :

Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-PE. Green line: Cells + Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE), (2.5 µg).

product information

CAT :

APR-008-PE

SKU :

APR-008-PE_15 mcl

Product Name :

Anti-P2X7 Receptor (extracellular)-PE Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR008

Homology :

Human, rat - identical; bovine - 14/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

R-Phycoerythrin (R-PE).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Product Page - Scientific background :

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis1-3.Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization, and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx, and cell phenotype4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X7 Conjugated to the Fluorescent Dye Phycoerythrin

Long Description :

Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples. Anti-P2X7 Receptor (extracellular)-PE Antibody (#APR-008-PE) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-PE

Positive Control :

Synonyms :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX7

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage