Anti-P2X7 Receptor (extracellular)-FITC Antibody | Alomone Labs APR-008-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X7 Receptor (extracellular)-FITC Antibody

catalog :

APR-008-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher
Rupert M, Bhattacharya A, Stillerova V, Jindrichova M, Mokdad A, Bou xe9 Grabot E, et al. Role of Conserved Residues and F322 in the Extracellular Vestibule of the Rat P2X7 Receptor in Its Expression, Function and Dye Uptake Ability. Int J Mol Sci. 2020;21: pubmed publisher
Bautista P xe9 rez R, P xe9 rez M xe9 ndez O, Cano Mart xed nez A, Pacheco U, Santamar xed a J, Rodr xed guez Iturbe F, et al. The Role of P2X7 Purinergic Receptors in the Renal Inflammation Associated with Angiotensin II-induced Hypertension. Int J Mol Sci. 2020;21: pubmed publisher
Semenova S, Shatrova A, Vassilieva I, Shamatova M, Pugovkina N, Negulyaev Y. Adenosine-5'-triphosphate suppresses proliferation and migration capacity of human endometrial stem cells. J Cell Mol Med. 2020;24:4580-4588 pubmed publisher
Zumerle S, Calì B, Munari F, Angioni R, Di Virgilio F, Molon B, et al. Intercellular Calcium Signaling Induced by ATP Potentiates Macrophage Phagocytosis. Cell Rep. 2019;27:1-10.e4 pubmed publisher
Grygorowicz T, Struzynska L. Early P2X7R-dependent activation of microglia during the asymptomatic phase of autoimmune encephalomyelitis. Inflammopharmacology. 2019;27:129-137 pubmed publisher
Fagerberg S, Patel P, Andersen L, Lui X, Donnino M, Praetorius H. Erythrocyte P2X1 receptor expression is correlated with change in haematocrit in patients admitted to the ICU with blood pathogen-positive sepsis. Crit Care. 2018;22:181 pubmed publisher
D Addio F, Vergani A, Potena L, Maestroni A, Usuelli V, Ben Nasr M, et al. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes. J Clin Invest. 2018;128:3490-3503 pubmed publisher
Amadio S, Parisi C, Piras E, Fabbrizio P, Apolloni S, Montilli C, et al. Modulation of P2X7 Receptor during Inflammation in Multiple Sclerosis. Front Immunol. 2017;8:1529 pubmed publisher
Luchting B, Heyn J, Woehrle T, Rachinger Adam B, Kreth S, Hinske L, et al. Differential expression of P2X7 receptor and IL-1β in nociceptive and neuropathic pain. J Neuroinflammation. 2016;13:100 pubmed publisher

image

image 1 :

Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells: Cell surface detection of P2RX7 in live intact mouse BV-2 microglia cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F), (5 µg).

image 2 :

Cell surface detection of P2RX7 inlive intact living Jurkat T-cells: - ___Unstained cells.___Cells +Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F) (10 g per 1x106 cells).

product information

CAT :

APR-008-F

SKU :

APR-008-F_15 mcl

Product Name :

Anti-P2X7 Receptor (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H244

Applications :

FC IC IF LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR008

Homology :

Human, rat - identical; bovine - 14/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor

Product Page - Scientific background :

The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4

Applications may also work in :

FC IC IF LCI

Supplier :

Alomone Labs

Target :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples. Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry and immunocytochemistry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX7

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC FC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage