Anti-P2Y4 Receptor Antibody | Alomone Labs APR-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2Y4 Receptor Antibody

catalog :

APR-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; fig 2	Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
	Gohar E, Kasztan M, Zhang S, Inscho E, Pollock D. Role for ovarian hormones in purinoceptor-dependent natriuresis. Biol Sex Differ. 2020;11:52 pubmed publisher
	Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
	Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed publisher
	Zhou F, Liu X, Gao L, Zhou X, Cao Q, Niu L, et al. HIV-1 Tat enhances purinergic P2Y4 receptor signaling to mediate inflammatory cytokine production and neuronal damage via PI3K/Akt and ERK MAPK pathways. J Neuroinflammation. 2019;16:71 pubmed publisher
	Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-P2Y4 Receptor Antibody (#APR-006), (1:300).2. Anti-P2Y4 Receptor Antibody, preincubated with P2Y4 Receptor Blocking Peptide (#BLP-PR006).

image 2 :

Expression of P2RY4 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-P2Y4 Receptor Antibody (#APR-006). A. P2RY4 (red) appears in astrocytic processes (arrows). B. In contrast, P2RY4 does not appear in Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2RY4 to astrocytic fibers.

image 3 :

Expression of P2RY4 in rat cerebellum - Immunohistochemical staining of rat cerebellumusingAnti-P2Y4 ReceptorAntibody (#APR-006). A. P2RY4 (red) appears in astrocytic processes (arrows). B.In contrast P2RY4 does not appearin Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2RY4 to astrocytic fibers.

product information

CAT :

APR-006

SKU :

APR-006-CF_0.2 ml

Product Name :

Anti-P2Y4 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O35811

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR006

Homology :

Mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4

Product Page - Scientific background :

The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4The P2Y4-receptor is highly expressed in the placenta and with lower levels in the lung and vascular smooth muscle.5The human P2Y4 receptor is highly selective to UTP while rat P2Y4 receptor is equally activated by both UTP and ATP and also by other triphosphate nucleotides such as Ap4A.5Recently it has been shown that P2Y4 receptor is involved in differentiation and cell death of human neuroblastoma SH-SY5Y cells.6

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor

Short Description :

A Rabbit Polyclonal Antibody to P2Y4 Receptor

Long Description :

Anti-P2Y4 Receptor Antibody (#APR-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2RY4 in rat, mouse, and human samples.

Negative Control :

BLP-PR006

Positive Control :

Synonyms :

P2Y purinoceptor 4, P2RY4, Uridine nucleotide receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RY4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:300

more info or order :

Alomone Labs product webpage