Anti-P2X5 Receptor Antibody | Alomone Labs APR-005 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X5 Receptor Antibody

catalog :

APR-005

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-P2X5 Receptor Antibody (#APR-005), (1:200). 2. Anti-P2X5 Receptor Antibody, preincubated with P2X5 Receptor Blocking Peptide (#BLP-PR005).

image 2 :

Expression of P2RX5 in rat spinal cord - Immunohistochemical staining of rat spinal cord frozen section using Anti-P2X5 Receptor Antibody (#APR-005), (1:100). P2RX5 staining (red) is specific for neurons in the anterior horn of the spinal cord. Hoechst 33342 is used as the counterstain.

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-P2X5 ReceptorAntibody (#APR-005)(1:200).2. Anti-P2X5 Receptor Antibodypreincubated with the control peptide antigen.

product information

CAT :

APR-005

SKU :

APR-005-CF_0.2 ml

Product Name :

Anti-P2X5 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51578

Applications :

IF IFC IHC WB

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR005

Homology :

Mouse - 13/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 of rat P2X5 receptor

Product Page - Scientific background :

The P2X5 receptor (P2RX5) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.1,2,3 All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The P2RX5 forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.4,6 The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia. The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland. Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.7-10

Applications may also work in :

IF IFC IHC WB

Supplier :

Alomone Labs

Target :

P2RX5, P2X purinoceptor 5

Short Description :

A Rabbit Polyclonal Antibody to P2X5 Receptor

Long Description :

Alomone Labs offers a highly specific antibody directed against an intracellular epitope at the C-terminus of the rat P2X5 receptor. Anti-P2X5 Receptor Antibody (#APR-005) can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize P2RX5 from rat and mouse samples. The antibody will not recognize the receptor from human samples.

Negative Control :

BLP-PR005

Positive Control :

Synonyms :

P2RX5, P2X purinoceptor 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX5

AB Specifictiy :

The antibody will not recognize the protein from human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage