Anti-P2X7 Receptor-ATTO Fluor-550 Antibody | Alomone Labs APR-004-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X7 Receptor-ATTO Fluor-550 Antibody

catalog :

APR-004-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Benzaquen J, Dit Hreich S, Heeke S, Juhel T, Lalvee S, Bauwens S, et al. P2RX7B is a new theranostic marker for lung adenocarcinoma patients. Theranostics. 2020;10:10849-10860 pubmed publisher
Yu X, Quan J, Long W, Chen H, Wang R, Guo J, et al. LL-37 inhibits LPS-induced inflammation and stimulates the osteogenic differentiation of BMSCs via P2X7 receptor and MAPK signaling pathway. Exp Cell Res. 2018;372:178-187 pubmed publisher

image

image 1 :

Multiplex staining of IBA1/AIF1 and P2X7 in rat brain. - Immunohistochemical staining of rat corpus callosum (CC) free floating frozen sections using Anti-IBA1/AIF1 Antibody (#ACS-010), (1:1000) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO) (1:60). A. IBA1/AIF1 immunoreactivity (green) appears in microglia (arrows). B. P2X7 immunostaining (red) appears in microglia (up-pointing arrows) and in other cell types in the corpus callosum (down-pointing arrows). C. Merged image of panels A and B demonstrates partial colocalization of both proteins. Nuclei are demonstrated using DAPI as the counterstain (blue).

image 2 :

Expression ofP2RX7in rat hippocampus - Immunohistochemical staining of rat hippocampus frozen section usingAnti-P2X7 Receptor Antibody (#APR-004-AO) (1:60). A. P2X7 staining (red) appears in the CA1 pyramidal (Pyr) and stratum radiatum (SR) layers in cells with glial morphology (arrows). B. DAPI is used as the counterstain (blue). C. Merged image of A and B.

image 3 :

Alomone Labs ML133 hydrochloride blocks Kir2.1 channels expressed in HEK293 cells. - Top: Representative Kir2.1 current traces before (blue) and after (red) application of 10 M ML133 hydrochloride (#M-275) recorded in pH 8.5 (left) or pH 7.4 (right). Currents were evoked from a holding potential of 0 mV by a 500 ms step to -100 mV followed by a 500 ms ramp from -100 mV to 100 mV.Bottom: Corresponding time course of Kir2.1 current inhibition by 10 M ML133 hydrochloride as indicated (bar) in pH 8.5 (left) or pH 7.4 (right).

product information

CAT :

APR-004-AO

SKU :

APR-004-AO_50 mcl

Product Name :

Anti-P2X7 Receptor-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64663

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR004

Homology :

Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor

Product Page - Scientific background :

The P2X7 purinergic receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family consists of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells, and microglia.The P2X7 receptor mediates the release of proinflammatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-P2X7 Receptor Antibody (#APR-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2X7 purinergic receptor from rat, mouse, and human samples. Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-P2X7 Receptor-ATTO Fluor-550 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX7

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-004), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:20-1:100

more info or order :

Alomone Labs product webpage