Anti-P2X2 Receptor Antibody | Alomone Labs APR-003 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X2 Receptor Antibody

catalog :

APR-003

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 19

Reference
Travers S, Kalyanasundar B, Breza J, Houser G, Klimovich C, TRAVERS J. Characteristics and Impact of the rNST GABA Network on Neural and Behavioral Taste Responses. Eneuro. 2022;9: pubmed publisher
Miyamoto K, Ishibashi Y, Akita T, Yamashita C. Systemic Delivery of hGhrelin Derivative by Lyophilizate for Dry Powder Inhalation System in Monkeys. Pharmaceutics. 2021;13: pubmed publisher
Yu T, Wilson C, Stratford J, Finger T. Genetic Deletion of TrpV1 and TrpA1 Does Not Alter Avoidance of or Patterns of Brainstem Activation to Citric Acid in Mice. Chem Senses. 2020;45:573-579 pubmed publisher
Khoo K, Galleano I, Gasparri F, Wieneke R, Harms H, Poulsen M, et al. Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing. Nat Commun. 2020;11:2284 pubmed publisher
Kalyanasundar B, Blonde G, Spector A, Travers S. Electrophysiological responses to sugars and amino acids in the nucleus of the solitary tract of type 1 taste receptor double-knockout mice. J Neurophysiol. 2020;123:843-859 pubmed publisher
Jeong J, Kim J, Hong H, Wangemann P, Marcus D, Jung J, et al. P2RX2 and P2RX4 receptors mediate cation absorption in transitional cells and supporting cells of the utricular macula. Hear Res. 2020;386:107860 pubmed publisher
George B, Swartz K, Li M. Hearing loss mutations alter the functional properties of human P2X2 receptor channels through distinct mechanisms. Proc Natl Acad Sci U S A. 2019;116:22862-22871 pubmed publisher
Deraos G, Kritsi E, Matsoukas M, Christopoulou K, Kalbacher H, Zoumpoulakis P, et al. Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82⁻98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II. Brain Sci. 2018;8: pubmed publisher
Sirko P, Gale J, Ashmore J. Intercellular Ca2+ signalling in the adult mouse cochlea. J Physiol. 2019;597:303-317 pubmed publisher
Takimoto Y, Ishida Y, Kondo M, Imai T, Hanada Y, Ozono Y, et al. P2X2 Receptor Deficiency in Mouse Vestibular End Organs Attenuates Vestibular Function. Neuroscience. 2018;386:41-50 pubmed publisher
Yoshida R, Shin M, Yasumatsu K, Takai S, Inoue M, Shigemura N, et al. The Role of Cholecystokinin in Peripheral Taste Signaling in Mice. Front Physiol. 2017;8:866 pubmed publisher
Sáez Orellana F, Fuentes Fuentes M, Godoy P, Silva Grecchi T, Panes J, Guzman L, et al. P2X receptor overexpression induced by soluble oligomers of amyloid beta peptide potentiates synaptic failure and neuronal dyshomeostasis in cellular models of Alzheimer's disease. Neuropharmacology. 2018;128:366-378 pubmed publisher
Gaillard D, Bowles S, Salcedo E, Xu M, Millar S, Barlow L. β-catenin is required for taste bud cell renewal and behavioral taste perception in adult mice. PLoS Genet. 2017;13:e1006990 pubmed publisher
Coddou C, Sandoval R, Castro P, Lazcano P, Hevia M, Rokic M, et al. Cyclin-dependent kinase 5 modulates the P2X2a receptor channel gating through phosphorylation of C-terminal threonine 372. Pain. 2017;158:2155-2168 pubmed publisher
Castillo Azofeifa D, Losacco J, Salcedo E, Golden E, Finger T, Barlow L. Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance. Development. 2017;144:3054-3065 pubmed publisher
Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
Stratford J, Thompson J, Finger T. Immunocytochemical organization and sour taste activation in the rostral nucleus of the solitary tract of mice. J Comp Neurol. 2017;525:271-290 pubmed publisher
de Baaij J, Kompatscher A, Viering D, Bos C, Bindels R, Hoenderop J. P2X6 Knockout Mice Exhibit Normal Electrolyte Homeostasis. PLoS ONE. 2016;11:e0156803 pubmed publisher
Breza J, Travers S. P2X2 Receptor Terminal Field Demarcates a "Transition Zone" for Gustatory and Mechanosensory Processing in the Mouse Nucleus Tractus Solitarius. Chem Senses. 2016;41:515-24 pubmed publisher

image

image 1 :

Western blot analysis of differentiated (lanes 1 and 3) and non-differentiated (lanes 2 and 4) PC12 cell lysates: - 1,2. Anti-P2X2 Receptor Antibody (#APR-003), (1:200).3,4. Anti-P2X2 Receptor Antibody, preincubated with P2X2 Receptor Blocking Peptide (#BLP-PR003).

image 2 :

Expression of P2X2 receptor in rat neocortex - Immunohistochemical staining of rat neocortex with Anti-P2X2 Receptor Antibody (#APR-003). P2X2 receptor (green) appears in axonal processes (arrows) that ascend toward the upper cortical layers (asterisk). DAPI is used as the counterstain (blue).

product information

CAT :

APR-003

SKU :

APR-003-CF_0.2 ml

Product Name :

Anti-P2X2 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49653

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR003

Homology :

Guinea pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor

Product Page - Scientific background :

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2X purinoceptor 2, P2RX2

Short Description :

A Rabbit Polyclonal Antibody to P2X2 Receptor

Long Description :

Anti-P2X2 Receptor Antibody (#APR-003) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X2 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR003

Positive Control :

Synonyms :

P2X purinoceptor 2, P2RX2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage