Anti-P2X2 Receptor-ATTO Fluor-594 Antibody | Alomone Labs APR-003-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X2 Receptor-ATTO Fluor-594 Antibody

catalog :

APR-003-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Jeong J, Kim J, Hong H, Wangemann P, Marcus D, Jung J, et al. P2RX2 and P2RX4 receptors mediate cation absorption in transitional cells and supporting cells of the utricular macula. Hear Res. 2020;386:107860 pubmed publisher
Takimoto Y, Ishida Y, Kondo M, Imai T, Hanada Y, Ozono Y, et al. P2X2 Receptor Deficiency in Mouse Vestibular End Organs Attenuates Vestibular Function. Neuroscience. 2018;386:41-50 pubmed publisher

image

image 1 :

Expression of P2RX2 in rat brain - Immunohistochemical staining of rat neocortex using Anti-P2X2 Receptor-ATTO Fluor-594 Antibody (#APR-003-AR). A. P2RX2 (red) is expressed in dendritic and axonal processes. B. Nucleus staining using DAPI as the counterstain (blue). C. Merge Images of A and B.

product information

CAT :

APR-003-AR

SKU :

APR-003-AR_50 mcl

Product Name :

Anti-P2X2 Receptor-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49653

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR003

Homology :

Guinea pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor

Product Page - Scientific background :

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subtypes has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found.10

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

P2X purinoceptor 2, P2RX2

Short Description :

A Rabbit Polyclonal Antibody to P2X2 Receptor Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-P2X2 Receptor Antibody (#APR-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2X2 receptor from rat, mouse, and human samples. Anti-P2X2 Receptor-ATTO Fluor-594 Antibody (#APR-003-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-P2X2 Receptor-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-PR003

Positive Control :

Synonyms :

P2X purinoceptor 2, P2RX2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APR-003), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage