Anti-P2X4 Receptor Antibody | Alomone Labs APR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X4 Receptor Antibody

catalog :

APR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Alomone Labs product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; loading ...; fig 2b immunohistochemistry; mouse	Yu W, Hill W, Robson S, Zeidel M. Role of P2X4 Receptor in Mouse Voiding Function. Sci Rep. 2018;8:1838 pubmed publisher
western blot; mouse; loading ...; fig 5d	Hayashi Y, Morinaga S, Zhang J, Satoh Y, Meredith A, Nakata T, et al. BK channels in microglia are required for morphine-induced hyperalgesia. Nat Commun. 2016;7:11697 pubmed publisher
western blot; mouse; 1:1000; fig 2	Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
	Fu X, Zhou G, Wu X, Xu C, Zhou H, Zhuang J, et al. Inhibition of P2X4R attenuates white matter injury in mice after intracerebral hemorrhage by regulating microglial phenotypes. J Neuroinflammation. 2021;18:184 pubmed publisher
	Xiang Z, Jiang X, Ji R, Yuan H. Enhanced expression of P2X4 purinoceptors in pyramidal neurons of the rat hippocampal CA1 region may be involved ischemia-reperfusion injury. Purinergic Signal. 2021;17:425-438 pubmed publisher
	Wang M, Cai X, Wang Y, Li S, Wang N, Sun R, et al. Astragalin Alleviates Neuropathic Pain by Suppressing P2X4-Mediated Signaling in the Dorsal Root Ganglia of Rats. Front Neurosci. 2020;14:570831 pubmed publisher
	Nguyen H, Di Lucente J, Chen Y, Cui Y, Ibrahim R, Pennington M, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4-mediated calcium entry in microglia. Glia. 2020;68:2377-2394 pubmed publisher
	Montilla A, Zabala A, Matute C, Domercq M. Functional and Metabolic Characterization of Microglia Culture in a Defined Medium. Front Cell Neurosci. 2020;14:22 pubmed publisher
	Jeong J, Kim J, Hong H, Wangemann P, Marcus D, Jung J, et al. P2RX2 and P2RX4 receptors mediate cation absorption in transitional cells and supporting cells of the utricular macula. Hear Res. 2020;386:107860 pubmed publisher
	Ribeiro D, Casarotto P, Staquini L, Pinto E Silva M, Biojone C, Wegener G, et al. Reduced P2X receptor levels are associated with antidepressant effect in the learned helplessness model. Peerj. 2019;7:e7834 pubmed publisher
	Arkhipov S, Potter D, Geurts A, Pavlov T. Knockout of P2rx7 purinergic receptor attenuates cyst growth in a rat model of ARPKD. Am J Physiol Renal Physiol. 2019;317:F1649-F1655 pubmed publisher
	Bragança B, Nogueira Marques S, Ferreirinha F, Fontes Sousa A, Correia de Sá P. The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy. Front Pharmacol. 2019;10:1103 pubmed publisher
	Zumerle S, Calì B, Munari F, Angioni R, Di Virgilio F, Molon B, et al. Intercellular Calcium Signaling Induced by ATP Potentiates Macrophage Phagocytosis. Cell Rep. 2019;27:1-10.e4 pubmed publisher
	Dhuna K, Felgate M, Bidula S, Walpole S, Bibic L, Cromer B, et al. Ginsenosides Act As Positive Modulators of P2X4 Receptors. Mol Pharmacol. 2019;95:210-221 pubmed publisher
	Palygin O, Ilatovskaya D, Levchenko V, Klemens C, Dissanayake L, Williams A, et al. Characterization of purinergic receptor expression in ARPKD cystic epithelia. Purinergic Signal. 2018;14:485-497 pubmed publisher
	Ali S, Turner J, Fountain S. Constitutive P2Y2 receptor activity regulates basal lipolysis in human adipocytes. J Cell Sci. 2018;131: pubmed publisher
	Su W, Wu F, Jin Z, Gu Y, Chen Y, Fei Y, et al. Overexpression of P2X4 receptor in Schwann cells promotes motor and sensory functional recovery and remyelination via BDNF secretion after nerve injury. Glia. 2019;67:78-90 pubmed publisher
	Yang R, Li L, Yuan H, Liu H, Gong Y, Zou L, et al. Quercetin relieved diabetic neuropathic pain by inhibiting upregulated P2X4 receptor in dorsal root ganglia. J Cell Physiol. 2019;234:2756-2764 pubmed publisher
	Ali S, Turner J, Fountain S. P2Y2 and P2Y6 receptor activation elicits intracellular calcium responses in human adipose-derived mesenchymal stromal cells. Purinergic Signal. 2018;14:371-384 pubmed publisher
	Xu F, Yang J, Lu F, Liu R, Zheng J, Zhang J, et al. Fast Green FCF Alleviates Pain Hypersensitivity and Down-Regulates the Levels of Spinal P2X4 Expression and Pro-inflammatory Cytokines in a Rodent Inflammatory Pain Model. Front Pharmacol. 2018;9:534 pubmed publisher
	Al Khalidi R, Panicucci C, Cox P, Chira N, Róg J, Young C, et al. Zidovudine ameliorates pathology in the mouse model of Duchenne muscular dystrophy via P2RX7 purinoceptor antagonism. Acta Neuropathol Commun. 2018;6:27 pubmed publisher
	Khoja S, Huynh N, Asatryan L, Jakowec M, Davies D. Reduced expression of purinergic P2X4 receptors increases voluntary ethanol intake in C57BL/6J mice. Alcohol. 2018;68:63-70 pubmed publisher
	Lalisse S, Hua J, Lenoir M, Linck N, Rassendren F, Ulmann L. Sensory neuronal P2RX4 receptors controls BDNF signaling in inflammatory pain. Sci Rep. 2018;8:964 pubmed publisher
	Green J, Souilhol C, Xanthis I, Martinez Campesino L, Bowden N, Evans P, et al. Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling. Cardiovasc Res. 2017;: pubmed publisher
	Verma R, Cronin C, Hudobenko J, Venna V, McCullough L, Liang B. Deletion of the P2X4 receptor is neuroprotective acutely, but induces a depressive phenotype during recovery from ischemic stroke. Brain Behav Immun. 2017;66:302-312 pubmed publisher
	Sander P, Mostafa H, Soboh A, Schneider J, Pala A, Baron A, et al. Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP. Oncotarget. 2017;8:35124-35137 pubmed publisher
	Shih H, Kuan Y, Shyu B. Targeting brain-derived neurotrophic factor in the medial thalamus for the treatment of central poststroke pain in a rodent model. Pain. 2017;158:1302-1313 pubmed publisher
	Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-P2X4 Receptor Antibody (#APR-002), (1:200).2. Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).

image 2 :

Expression of P2RX4 in rat brain - Immunohistochemical staining of rat brain red nucleus using Anti-P2X4 Receptor Antibody (#APR-002). A. P2RX4 (green) appears in fibers surrounding cell shapes (arrows). B. Calbindin 28K (red) appears in large neurons. C. Merge of P2RX4 and Calbindin 28K suggests variable density of P2RX4 expressing fibers on red nucleus neurons. DAPI is used as the counterstain (blue).

image 3 :

Expression of P2X4 receptor in lysosomes. - Immunocytochemical staining of fixed and permeated COS1 cells using Anti-P2X4 Receptor Antibody (#APR-002). A. P2X4 staining (green) co-localizes with Lamp1 (red) a lysosomal marker. B. Fractionation of COS1 cells shows that P2X4 receptor is detected in the lysosome fraction along with Lamp1. C. Similar results are obtained when P2X4 is heterologously expressed.Adapted from Huang P. et al. (2014) with permission of The American Society for Biochemistry and Molecular Biology.

product information

CAT :

APR-002

SKU :

APR-002-CF_0.2 ml

Product Name :

Anti-P2X4 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51577

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR002

Homology :

Mouse - identical; human - 15/19 amino acid residues identical; chicken - 11/12 amino acid residues identical; Xenopus - 11/11 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 receptor

Product Page - Scientific background :

The P2X purinergic receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop.All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2RX4, P2X purinoceptor 4, ATP receptor

Short Description :

A Rabbit Polyclonal Antibody to P2X4 Receptor

Long Description :

Anti-P2X4 Receptor Antibody (#APR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, immunoprecipitation, and indirect flow cytometry applications. It has been designed to recognize P2X4 purinergic receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR002

Positive Control :

Synonyms :

P2RX4, P2X purinoceptor 4, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage